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To determine the level of antibodies against meningococcal A polysaccharide (<jats:styled-content style=\"fixed-case\">APS<\/jats:styled-content>) that correlates with protection against MenA disease in the <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>frican meningitis belt, it may be important to consider antibody avidity along with quantity. In this study, two <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> methods using the chaotropic agent ammonium thiocyanate were compared and employed to measure avidity indexes (<jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>) of IgG antibodies against <jats:styled-content style=\"fixed-case\">APS<\/jats:styled-content> in controls and in acute and convalescent sera from Ethiopian meningococcal patients. High statistical correlations between the <jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>s determined by the two methods were observed. The geometric mean <jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">GMAI<\/jats:styled-content>) increased with time from acute to convalescent sera indicating affinity maturation. <jats:styled-content style=\"fixed-case\">GMAI<\/jats:styled-content> was significantly higher in convalescent sera from the MenA patients and in sera from the controls than in acute sera from patients with meningococcal disease. A significant correlation between serum bactericidal activity titres (<jats:styled-content style=\"fixed-case\">SBA<\/jats:styled-content>) and concentration of IgG antibodies against <jats:styled-content style=\"fixed-case\">APS<\/jats:styled-content> was observed; however, our results did not indicate that determination of antibody avidities by the thiocyanate elution method gave a better correlation with <jats:styled-content style=\"fixed-case\">SBA<\/jats:styled-content> than anti\u2010<jats:styled-content style=\"fixed-case\">APS<\/jats:styled-content> IgG concentrations determined by the standard <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> method.<\/jats:p>","DOI":"10.1111\/sji.12150","type":"journal-article","created":{"date-parts":[[2014,1,2]],"date-time":"2014-01-02T18:35:00Z","timestamp":1388687700000},"page":"267-275","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Avidity of IgG Antibodies Against Meningococcal Serogroup A Polysaccharide and Correlations with Bactericidal Activity in Sera From Meningitis Patients and Controls From <scp>E<\/scp>thiopia"],"prefix":"10.1111","volume":"79","author":[{"given":"M.","family":"Medhane","sequence":"first","affiliation":[{"name":"Department of Biology Addis Ababa University Addis Ababa Ethiopia"},{"name":"Armauer Hansen Research Institute Addis Ababa Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Tunheim","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Immunology Norwegian Institute of Public Health Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. 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