{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:37:31Z","timestamp":1791247051589,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,8,21]],"date-time":"2014-08-21T00:00:00Z","timestamp":1408579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The immunological paradox of pregnancy, when maternal immune system recognizes but does not reject the semiallogenic foetus, is not yet fully understood. The aim of this work was to detail the mechanisms of the interaction of decidual <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>56+ <jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content>, infiltrating the maternal part of placenta, and trophoblast cells of foetal origin. Samples of the endometrial tissue from 13 healthy non\u2010pregnant women, 37 placentas, obtained after medical abortion of viable pregnancy at 7\u201310\u00a0weeks of gestation, and 26 samples of placentas from first\u2010trimester recurrent spontaneous abortion (<jats:styled-content style=\"fixed-case\">RSA<\/jats:styled-content>) were used as the material for investigation. Phenotype of <jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content> was assessed by flow cytometry. The influence of trophoblast cells upon <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content><jats:italic>\u03b3<\/jats:italic> and GrB <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s expression by <jats:styled-content style=\"fixed-case\">dNK<\/jats:styled-content> was investigated by <jats:styled-content style=\"fixed-case\">RT\u2010PCR<\/jats:styled-content>. The influence of <jats:styled-content style=\"fixed-case\">dNK<\/jats:styled-content> upon trophoblast cells migration and invasion was studied using collagen and Matrigel systems. In <jats:styled-content style=\"fixed-case\">RSA<\/jats:styled-content> group comparing to the normal pregnancy, the decrease of <jats:styled-content style=\"fixed-case\">dNK<\/jats:styled-content> with inhibitory receptors (<jats:styled-content style=\"fixed-case\">NKG<\/jats:styled-content>2A) and elevation of activated <jats:styled-content style=\"fixed-case\">dNK<\/jats:styled-content> were seen. In normal pregnancy, but not in <jats:styled-content style=\"fixed-case\">RSA<\/jats:styled-content>, trophoblast cells increased the expression of <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content><jats:italic>\u03b3<\/jats:italic> and GrB <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s by <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>56+ <jats:styled-content style=\"fixed-case\">dNK<\/jats:styled-content>. Both in normal and <jats:styled-content style=\"fixed-case\">RSA<\/jats:styled-content> pregnancy, <jats:styled-content style=\"fixed-case\">dNK<\/jats:styled-content> inhibited the migration and invasion of trophoblast cells. Initially, low invasive and migration capacities of trophoblast cells were seen during <jats:styled-content style=\"fixed-case\">RSA<\/jats:styled-content>. Thus, unbalanced activation of <jats:styled-content style=\"fixed-case\">dNK<\/jats:styled-content> can lead to the impairment of <jats:styled-content style=\"fixed-case\">dNK<\/jats:styled-content> and trophoblast cells interaction during <jats:styled-content style=\"fixed-case\">RSA<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/sji.12196","type":"journal-article","created":{"date-parts":[[2014,6,9]],"date-time":"2014-06-09T10:47:43Z","timestamp":1402310863000},"page":"198-208","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Interaction of Decidual <scp>CD<\/scp>56+ <scp>NK<\/scp> with Trophoblast Cells during Normal Pregnancy and Recurrent Spontaneous Abortion at Early Term of Gestation"],"prefix":"10.1111","volume":"80","author":[{"given":"N.","family":"Sotnikova","sequence":"first","affiliation":[{"name":"Federal State Research Institute of Maternity and Childhood named V.N. 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