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The immune system has to recognize syngeneic tumour cells and has to be primed and respond in an adequate manner. Priming of a leukaemia\u2010specific immune response is a crucial step in tumour immunology that can mislead to tumour tolerance either by T cell ignorance, deletion or Treg induction. To resemble the situation of acute lymphoblastic leukaemia (<jats:styled-content style=\"fixed-case\">ALL<\/jats:styled-content>) in patients, we used the murine <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c model with syngeneic <jats:styled-content style=\"fixed-case\">BM<\/jats:styled-content>185 tumour cells. We established a tumour cell line that expresses the neo\u2010antigen ovalbumin (<jats:styled-content style=\"fixed-case\">BM<\/jats:styled-content>185\u2010<jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>) to allow the application of T cell receptor transgenic, antigen\u2010specific <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T cells. Here, we demonstrate that effective anti\u2010<jats:styled-content style=\"fixed-case\">ALL<\/jats:styled-content> immunity can be established by <jats:italic>in vivo<\/jats:italic> priming of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T cells that is sufficient to differentiate into effector cells. Yet they failed to control tumour alone, but initiated a Th1 response. An efficient tumour clearance was dependent on both antigen\u2010specific <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T cells and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> effector T cells from the endogenous repertoire. The tolerogeneic milieu was characterized by increased Tregs numbers and elevated <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 level. Tregs hamper effective antitumour immune response, but their depletion did not result in reduced tumour growth. In contrast, neutralization of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 improved median mouse survival. Future therapies should focus on establishing a strong <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4+ T cells response, either by adjuvant or by adoptive transfer.<\/jats:p>","DOI":"10.1111\/sji.12198","type":"journal-article","created":{"date-parts":[[2014,6,9]],"date-time":"2014-06-09T10:47:44Z","timestamp":1402310864000},"page":"161-168","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Naive Tumour\u2010Specific CD4<sup>+<\/sup> T Cells were Efficiently Primed in Acute Lymphoblastic Leukaemia"],"prefix":"10.1111","volume":"80","author":[{"given":"F.","family":"Noyan","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Hepatology and Endocrinology Hannover Medical School Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Lieke","sequence":"additional","affiliation":[{"name":"ReMediES Department of General\u2010, Visceral\u2010 and Transplantation Surgery Hannover Medical School Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Taubert","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hepatology and Endocrinology Hannover Medical School Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Sievers","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hepatology and Endocrinology Hannover Medical School Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Dywicki","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hepatology and Endocrinology Hannover Medical School Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Hapke","sequence":"additional","affiliation":[{"name":"Department Pediatric Hematology\/Oncology Hannover Medical School Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. S.","family":"Falk","sequence":"additional","affiliation":[{"name":"Institute of Transplantation Immunology and Integrated Research and Treatment Center Transplantation (IFB\u2010Tx) Hannover Medical School Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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