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Several vaccine candidates were identified from infective third\u2010stage larvae (L3) which involves in the critical transition from arthropod to human. Hitherto studies of these antigens in combination with alum adjuvant have shown to elicit its characteristic Th2 responses. Inulin is a safe, non\u2010toxic adjuvant that principally stimulates the innate immune response through the alternative complement pathway. In the present study, the immune response elicited by inulin and alum as adjuvants were compared with filarial antigens from different aetiological agents: secreted larval acidic protein 1 (<jats:styled-content style=\"fixed-case\">SLAP<\/jats:styled-content>1) from <jats:italic><jats:styled-content style=\"fixed-case\">O<\/jats:styled-content>nchocerca volvulus<\/jats:italic> and venom allergen homologue (<jats:styled-content style=\"fixed-case\">VAH<\/jats:styled-content>) from <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>rugia malayi<\/jats:italic> as single or as cocktail vaccines in mice model. The study revealed that inulin can induce better humoral response against these antigens than alum adjuvant. Antibody isotyping disclosed inulin's ability to elevate the levels of IgG2a and IgG3 antibodies which mediates in complement\u2010dependent cytotoxicity and antibody\u2010dependent cell\u2010mediated cytotoxicity (<jats:styled-content style=\"fixed-case\">ADCC<\/jats:styled-content>), respectively, in mice. Splenocyte analysis showed that T cells prestimulated with inulin have higher stimulation index (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) than alum except for Bm<jats:styled-content style=\"fixed-case\">VAH<\/jats:styled-content> antigen. <jats:italic>In vitro <\/jats:italic><jats:styled-content style=\"fixed-case\">ADCC<\/jats:styled-content> assay showed that inulin formulation had induced higher cytotoxicity with filarial antigens (as single <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01 and as cocktail <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05, respectively) than alum. The results had confirmed the capability of inulin to deplete the levels of Treg and brought a balance in Th1\/Th2 arms against filarial antigens in mice.<\/jats:p>","DOI":"10.1111\/sji.12208","type":"journal-article","created":{"date-parts":[[2014,9,16]],"date-time":"2014-09-16T09:48:43Z","timestamp":1410860923000},"page":"261-270","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of Immune Response Elicited by Inulin as an Adjuvant with Filarial Antigens in Mice Model"],"prefix":"10.1111","volume":"80","author":[{"given":"N.","family":"Mahalakshmi","sequence":"first","affiliation":[{"name":"Centre for Biotechnology Anna University Guindy Chennai Tamilnadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Aparnaa","sequence":"additional","affiliation":[{"name":"Centre for Biotechnology Anna University Guindy Chennai Tamilnadu 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