{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:26:16Z","timestamp":1790515576251,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,2,24]],"date-time":"2015-02-24T00:00:00Z","timestamp":1424736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The genetically modified (<jats:styled-content style=\"fixed-case\">GM<\/jats:styled-content>) maize event <jats:styled-content style=\"fixed-case\">MON<\/jats:styled-content>810 has been inserted with a processed version of the transgene, <jats:italic>cry1Ab<\/jats:italic>, derived from the soil bacterium <jats:italic>Bacillus thuringiensis<\/jats:italic> (Bt) to express proteins with insecticidal properties. Such proteins may introduce new allergens and also act as adjuvants that promote allergic responses. While focus has been on safe consumption and hence the oral exposure to <jats:styled-content style=\"fixed-case\">GM<\/jats:styled-content> food and feed, little is known regarding inhalation of pollen and desiccated airborne plant material from <jats:styled-content style=\"fixed-case\">GM<\/jats:styled-content> crops. The aim of this study was to investigate whether plant material from the Cry1Ab\u2010expressing maize variety <jats:styled-content style=\"fixed-case\">MON<\/jats:styled-content>810, or trypsin\u2010activated Cry1Ab (trypCry1Ab) protein produced in recombinant bacteria, may act as adjuvants against the allergen ovalbumin (<jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>) in a mouse model of airway allergy. A clear proallergic adjuvant effect of the mucosal adjuvant cholera toxin (<jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content>) was demonstrated, determined as increased specific IgE, eosinophils and Th2 cytokines in <jats:styled-content style=\"fixed-case\">MLN<\/jats:styled-content> cell supernates, while no elevation in <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>\u2010specific antibodies or cytokine release from <jats:styled-content style=\"fixed-case\">MLN<\/jats:styled-content> cells after stimulation with <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content> were observed in mice receiving Cry1Ab\u2010containing plant materials or the trypCry1Ab protein. Our data suggest that Cry1Ab proteins had no detectable systemic adjuvant effect in mice after airway exposure. Further experiments with purified plant proteins, as well as long\u2010term exposures needs be conducted to further evaluate exposures experienced in real\u2010life situations.<\/jats:p>","DOI":"10.1111\/sji.12269","type":"journal-article","created":{"date-parts":[[2015,1,6]],"date-time":"2015-01-06T13:18:07Z","timestamp":1420550287000},"page":"192-200","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Cry1Ab Protein from <i>Bacillus thuringiensis<\/i> and <scp>MON<\/scp>810 <i>cry1Ab<\/i>\u2010transgenic Maize Exerts No Adjuvant Effect After Airway Exposure"],"prefix":"10.1111","volume":"81","author":[{"given":"M.","family":"Andreassen","sequence":"first","affiliation":[{"name":"Gen\u00d8k \u2010 Centre for Biosafety Troms\u00f8 Norway"},{"name":"Norwegian Institute of Public Health Oslo Norway"},{"name":"UiT The Arctic University of Norway Troms\u00f8 Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"B\u00f8hn","sequence":"additional","affiliation":[{"name":"Gen\u00d8k \u2010 Centre for Biosafety Troms\u00f8 Norway"},{"name":"UiT The Arctic University of Norway Troms\u00f8 Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.\u2010G.","family":"Wikmark","sequence":"additional","affiliation":[{"name":"Gen\u00d8k \u2010 Centre for Biosafety Troms\u00f8 Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Van den Berg","sequence":"additional","affiliation":[{"name":"North\u2010West University Potchefstroom South\u2010Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"L\u00f8vik","sequence":"additional","affiliation":[{"name":"Norwegian University of Science and Technology Trondheim Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Traavik","sequence":"additional","affiliation":[{"name":"Gen\u00d8k \u2010 Centre for Biosafety Troms\u00f8 Norway"},{"name":"UiT The Arctic University of Norway Troms\u00f8 Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U. 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