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One of the possible approaches is the early active immunization of risk patients and animal stocks to prevent <jats:italic>S.\u00a0aureus<\/jats:italic> infections. Based on a <jats:italic>S.\u00a0aureus<\/jats:italic> proteome screen with <jats:italic>S.\u00a0aureus<\/jats:italic>\u2010specific human antiserum, we have previously identified several anchorless cell wall proteins to be used as novel vaccine candidates. To develop an efficient anti\u2010<jats:italic>S.\u00a0aureus<\/jats:italic> vaccine, the supplemented adjuvants Montanide<jats:sup>\u2122<\/jats:sup> <jats:styled-content style=\"fixed-case\">ISA<\/jats:styled-content> 71 <jats:styled-content style=\"fixed-case\">VG<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ISA<\/jats:styled-content> 206 were compared to Freund's adjuvant in terms of handling, induction of cytokine profile, triggering antigen\u2010specific immunoglobulin production of different IgG subclasses and provision of increased survival rates in our <jats:italic>S.\u00a0aureus<\/jats:italic> sepsis mouse model. Immunization with <jats:styled-content style=\"fixed-case\">ISA<\/jats:styled-content> 71 <jats:styled-content style=\"fixed-case\">VG<\/jats:styled-content> in comparison with Freund's adjuvant induced slightly delayed but comparably strong increase of antigen\u2010specific antibody titres and conferred protective effect against <jats:italic>S.\u00a0aureus<\/jats:italic> challenge. In contrast using <jats:styled-content style=\"fixed-case\">ISA<\/jats:styled-content> 206 as adjuvant, significantly lower IgG titres and consequently, no protective effect against <jats:italic>S.\u00a0aureus<\/jats:italic> infection were observed. Handling and tolerability of the Montanide is superior to Freund's adjuvant. Montanide<jats:sup>\u2122<\/jats:sup> <jats:styled-content style=\"fixed-case\">ISA<\/jats:styled-content> 71 <jats:styled-content style=\"fixed-case\">VG<\/jats:styled-content> can serve as an effective adjuvant replacement for Freund's adjuvant in research with a prospective usage in animal and human vaccines against bacterial pathogens.<\/jats:p>","DOI":"10.1111\/sji.12279","type":"journal-article","created":{"date-parts":[[2015,2,18]],"date-time":"2015-02-18T10:00:29Z","timestamp":1424253629000},"page":"291-297","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Montanide <scp>ISA<\/scp> 71 <scp>VG<\/scp> is Advantageous to Freund's Adjuvant in Immunization Against <i>S.\u00a0aureus<\/i> Infection of Mice"],"prefix":"10.1111","volume":"81","author":[{"given":"A.","family":"Klimka","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology Immunology and Hygiene University Hospital Cologne 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