{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T17:04:53Z","timestamp":1780506293738,"version":"3.54.1"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,9,10]],"date-time":"2015-09-10T00:00:00Z","timestamp":1441843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology of Japan","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ministry of Health, Labor and Welfare of Japan"},{"name":"Nichias Corporation"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2015,10]]},"abstract":"Abstract<\/jats:title>DNA<\/jats:styled-content>\u2010mediated immunization of a tumour antigen is a possible immunotherapy for cancer, and interleukin (IL<\/jats:styled-content>)\u201027 has diverse functions in adaptive immunity. In this study, we examined whether IL<\/jats:styled-content>\u201027 DNA<\/jats:styled-content> administration enhanced antitumour effects in mice vaccinated with DNA<\/jats:styled-content> encoding a putative tumour antigen, \u03b2<\/jats:italic>\u2010galactosidase (\u03b2<\/jats:italic>\u2010gal). An intramuscular injection of cardiotoxin before DNA<\/jats:styled-content> administration facilitated the exogenous gene expression. In mice received \u03b2<\/jats:italic>\u2010gal and IL<\/jats:styled-content>\u201027 DNA<\/jats:styled-content>, growth of \u03b2<\/jats:italic>\u2010gal\u2010positive P815 tumours was retarded and survival of the mice was prolonged. Development of \u03b2<\/jats:italic>\u2010gal\u2010positive Colon 26 tumours was suppressed by vaccination of \u03b2<\/jats:italic>\u2010gal DNA<\/jats:styled-content> and further inhibited by additional IL<\/jats:styled-content>\u201027 DNA<\/jats:styled-content> administration or IL<\/jats:styled-content>\u201012 family cytokines. Nevertheless, a population of \u03b2<\/jats:italic>\u2010gal\u2010specific CD<\/jats:styled-content>8+<\/jats:sup> T cells did not increase, and production of anti\u2010\u03b2<\/jats:italic>\u2010gal antibody was not enhanced by IL<\/jats:styled-content>\u201027 DNA<\/jats:styled-content> administration. Spleen cells from mice bearing IL<\/jats:styled-content>\u201027\u2010expressing Colon 26 tumours showed greater YAC<\/jats:styled-content>\u20101\u2010targeted cytotoxicity although CD<\/jats:styled-content>3\u2212<\/jats:sup>\/DX<\/jats:styled-content>5+<\/jats:sup> natural killer (NK<\/jats:styled-content>) cell numbers remained unchanged. Recombinant IL<\/jats:styled-content>\u201027 enhanced YAC<\/jats:styled-content>\u20101\u2010targeted cytotoxicity of IL<\/jats:styled-content>\u20102\u2010primed splenic NK<\/jats:styled-content> cells and augmented a phosphorylation of signal transducer and activator of transcription 3 and an expression of perforin. These data collectively indicate that IL<\/jats:styled-content>\u201027 DNA<\/jats:styled-content> administration activates NK<\/jats:styled-content> cells and augments vaccination effects of DNA<\/jats:styled-content> encoding a tumour antigen through non\u2010adaptive immune responses.<\/jats:p>","DOI":"10.1111\/sji.12321","type":"journal-article","created":{"date-parts":[[2015,6,20]],"date-time":"2015-06-20T01:11:27Z","timestamp":1434762687000},"page":"320-327","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Administration of DNA<\/scp> Encoding the Interleukin\u201027 Gene Augments Antitumour Responses through Non\u2010adaptive Immunity"],"prefix":"10.1111","volume":"82","author":[{"given":"Q.","family":"Li","sequence":"first","affiliation":[{"name":"Division of Pathology and Cell Therapy Chiba Cancer Center Research Institute Chuo\u2010ku Chiba Japan"},{"name":"Department of Immunology Hebei Medical University 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