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Exosomes are intraluminal nanovesicles generated from multivesicular bodies, a late endosomal compartment. Cancer cells release exosomes that influence their proximate and distant environment to facilitate angiogenesis, metastatic dissemination and immune escape. Cancer\u2010derived vesicles may also trigger an anti\u2010tumour response by transferring tumour antigens to immune cells. We wanted to investigate whether differentiation and maturation of myeloid cells changes their capacity to take up cancer\u2010derived extracellular vesicles (<jats:styled-content style=\"fixed-case\">EV<\/jats:styled-content>). We compared the efficiency of vesicle uptake by monocytes, macrophages and dendritic cells. To visualize and quantify the cellular uptake, <jats:styled-content style=\"fixed-case\">EV<\/jats:styled-content> were labelled with two different dyes, carboxyfluoresceine diacetate succinimidyl\u2010ester (<jats:styled-content style=\"fixed-case\">CFSE<\/jats:styled-content>), or <jats:styled-content style=\"fixed-case\">DSSN<\/jats:styled-content>+, a water soluble distyrylstilbene oligoelectrolyte which preferentially intercalates into the cell membrane. With the help of cytokines, <jats:styled-content style=\"fixed-case\">THP<\/jats:styled-content>\u20101 monocytes were differentiated into immature or mature dendritic cells, or macrophages. <jats:styled-content style=\"fixed-case\">EV<\/jats:styled-content> uptake was monitored by flow cytometry and immunofluorescence microscopy. The results show that macrophages and mature dendritic cells acquired stronger fluorescence transferred by <jats:styled-content style=\"fixed-case\">EV<\/jats:styled-content> than monocytes or immature dendritic cells indicating that the differentiation status influences the efficiency of <jats:styled-content style=\"fixed-case\">EV<\/jats:styled-content> uptake.<\/jats:p>","DOI":"10.1111\/sji.12371","type":"journal-article","created":{"date-parts":[[2015,9,2]],"date-time":"2015-09-02T13:50:39Z","timestamp":1441201839000},"page":"506-514","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["The Uptake of Extracellular Vesicles is Affected by the Differentiation Status of Myeloid Cells"],"prefix":"10.1111","volume":"82","author":[{"given":"L.","family":"Czernek","sequence":"first","affiliation":[{"name":"Department of Bioorganic Chemistry Centre of Molecular and Macromolecular Studies Polish Academy of Sciences Lodz 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