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Three successive oral administrations of LTSH\u0394<jats:italic>stx<\/jats:italic> to female adult mice produced comprehensive passive heterologous protection in their offspring against challenge with wild\u2010type shigellae. Production of NO and different cytokines such asIL\u201012p70, IL\u20101\u03b2 and IL\u201023 in peritoneal mice macrophages indicated that LTSH\u0394<jats:italic>stx<\/jats:italic> induced innate and adaptive immunity in mice. Furthermore, production of IFN\u2010\u03b3, IL\u201010 and IL\u201017 in LTSH\u2010primed splenic CD4+ T cell suggested that LTSH\u0394<jats:italic>stx<\/jats:italic> may induce Th1 and Th17 cell\u2010mediated immune responses. Exponential increase of the serum IgG and IgA titre against whole shigellae was observed in immunized adult mice during and after the immunization with the highest peak on day 35. Antigen\u2010specific sIgA was also determined from intestinal lavage of immunized mice. The stomach extracts of neonates from immunized mice, mainly containing mother's milk, contained significant levels of anti\u2010LTSH\u0394<jats:italic>stx<\/jats:italic> immunoglobulin. These studies suggest that the LTSH\u0394<jats:italic>stx<\/jats:italic> could be a new live oral vaccine candidate against shigellosis in the near future.<\/jats:p>","DOI":"10.1111\/sji.12394","type":"journal-article","created":{"date-parts":[[2015,10,19]],"date-time":"2015-10-19T08:21:35Z","timestamp":1445242895000},"page":"92-101","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Immunization of Mice with a Live Transconjugant Shigella Hybrid Strain Induced Th1 and Th17 Cell\u2010Mediated Immune Responses and Confirmed Passive Protection Against Heterologous Shigellae"],"prefix":"10.1111","volume":"83","author":[{"given":"D.","family":"Nag","sequence":"first","affiliation":[{"name":"Division of Bacteriology National Institute of Cholera and Enteric Diseases Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Koley","sequence":"additional","affiliation":[{"name":"Division of Bacteriology National Institute of Cholera and Enteric Diseases Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Sinha","sequence":"additional","affiliation":[{"name":"Division of Bacteriology National Institute of Cholera and Enteric Diseases Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Division of Bacteriology National Institute of Cholera and Enteric Diseases Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Sarkar","sequence":"additional","affiliation":[{"name":"Division of Bacteriology National Institute of Cholera and Enteric Diseases Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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