{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T12:34:46Z","timestamp":1769603686471,"version":"3.49.0"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,3,11]],"date-time":"2016-03-11T00:00:00Z","timestamp":1457654400000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004257","name":"Helse Vest","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004257","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Patients with primary Sj\u00f6gren's syndrome (<jats:styled-content style=\"fixed-case\">pSS<\/jats:styled-content>) have an increased risk of developing lymphomas, particularly the subtype <jats:italic>mucosa\u2010associated lymphoid tissue<\/jats:italic> (<jats:styled-content style=\"fixed-case\">MALT<\/jats:styled-content>) lymphoma. Chronic antigen stimulation and increased activation of nuclear factor\u2010<jats:italic>\u03ba<\/jats:italic>B (<jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010<jats:italic>\u03ba<\/jats:italic>B) are important factors for the pathogenesis of <jats:styled-content style=\"fixed-case\">MALT<\/jats:styled-content> lymphomas. Protein A20 is an inhibitor of <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010<jats:italic>\u03ba<\/jats:italic>B. A recent study of <jats:styled-content style=\"fixed-case\">pSS<\/jats:styled-content>\u2010associated <jats:styled-content style=\"fixed-case\">MALT<\/jats:styled-content> lymphomas identified potential functional abnormalities in the <jats:italic><jats:styled-content style=\"fixed-case\">TNFAIP<\/jats:styled-content>3<\/jats:italic> gene, which encodes protein A20. The present study aimed to assess protein A20 by immunohistochemistry (<jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content>) in minor salivary glands (<jats:styled-content style=\"fixed-case\">MSG<\/jats:styled-content>s) and lymphoma tissue sections of patients with <jats:styled-content style=\"fixed-case\">pSS<\/jats:styled-content> and investigate a potential association with lymphoma development. Protein A20 staining in lymphocytes was scored in four categories (0\u00a0=\u00a0negative, 1\u00a0=\u00a0weak, 2\u00a0=\u00a0moderate and 3\u00a0=\u00a0strong). For statistical purposes, these scores were simplified into negative (scores 0\u20131) and positive (scores 2\u20133). We investigated associations between protein A20\u2010staining, focus scores, germinal centre (<jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>)\u2010like structures and monoclonal B\u2010cell infiltration in <jats:styled-content style=\"fixed-case\">MSG<\/jats:styled-content>s. <jats:styled-content style=\"fixed-case\">MSG<\/jats:styled-content> protein A20 staining was weaker in <jats:styled-content style=\"fixed-case\">pSS<\/jats:styled-content> patients with lymphomas than in those without lymphomas (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.01). Weak protein A20 staining was also highly associated with a lack of <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> formation (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01). Finally, weaker A20 staining was observed in the majority of <jats:styled-content style=\"fixed-case\">pSS<\/jats:styled-content>\u2010associated <jats:styled-content style=\"fixed-case\">MALT<\/jats:styled-content> lymphoma tissues. In conclusion, we found absent or weak protein A20 immunoreactivity in <jats:styled-content style=\"fixed-case\">MSG<\/jats:styled-content>s of patients with <jats:styled-content style=\"fixed-case\">pSS<\/jats:styled-content> with lymphomas. This finding indicates that protein A20 downregulation in lymphocytes might be a mechanism underlying lymphoma genesis in patients with <jats:styled-content style=\"fixed-case\">pSS<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/sji.12405","type":"journal-article","created":{"date-parts":[[2015,12,18]],"date-time":"2015-12-18T07:06:45Z","timestamp":1450422405000},"page":"181-187","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Low Protein A20 in Minor Salivary Glands is Associated with Lymphoma in Primary Sj\u00f6gren's Syndrome"],"prefix":"10.1111","volume":"83","author":[{"given":"S. J.","family":"Johnsen","sequence":"first","affiliation":[{"name":"Clinical Immunology Unit Department of Internal Medicine Stavanger University Hospital Stavanger Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Gudlaugsson","sequence":"additional","affiliation":[{"name":"Department of Pathology Stavanger University Hospital Stavanger Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Skaland","sequence":"additional","affiliation":[{"name":"Department of Pathology Stavanger University Hospital Stavanger Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. A. M.","family":"Janssen","sequence":"additional","affiliation":[{"name":"Department of Pathology Stavanger University Hospital Stavanger Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. V.","family":"Jonsson","sequence":"additional","affiliation":[{"name":"Section\u00a0for Oral and Maxillofacial Radiology Department of Clinical Dentistry University of Bergen Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Helgeland","sequence":"additional","affiliation":[{"name":"Department of Pathology Haukeland University Hospital Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Berget","sequence":"additional","affiliation":[{"name":"The Gade Laboratory for Pathology Department of Clinical Medicine University of Bergen Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Jonsson","sequence":"additional","affiliation":[{"name":"Broegelmann Research Laboratory Department of Clinical Science University of Bergen Bergen Norway"},{"name":"Department of Rheumatology Haukeland University Hospital Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Omdal","sequence":"additional","affiliation":[{"name":"Clinical Immunology Unit Department of Internal Medicine Stavanger University Hospital Stavanger Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,3,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2013.278448"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2011.06.007"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2012.10.006"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920112802273100"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2005.041186"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/acr.21887"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2010.144782"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.131527"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)92035-Z"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/sji.12101"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.005983"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2009.05.007"},{"key":"e_1_2_7_14_1","article-title":"Roles of A20 in autoimmune diseases","author":"Zhang M","year":"2015","journal-title":"Immunol Res"},{"key":"e_1_2_7_15_1","first-page":"708","article-title":"Functional evaluation of TNFAIP3 (A20) in rheumatoid arthritis","volume":"28","author":"Elsby LM","year":"2010","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/492872"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13322"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.27545"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2775"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07968"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-12-194852"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-08-174110"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07969"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-05-503383"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.61.6.554"},{"key":"e_1_2_7_26_1","volume-title":"Pathology and Genetics of Tumours of Haematopoietic and Lymphoid Tissues. World Health Organization Classification of Tumours","author":"Jaffe ESHN","year":"2001"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/gene.2010.64"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2792"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5488.2350"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-09-306019"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.07.017"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-011-0821-3"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803588"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2013.12.001"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.27564"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3313"}],"container-title":["Scandinavian Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fsji.12405","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/sji.12405","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T09:11:48Z","timestamp":1696151508000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/sji.12405"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3]]},"references-count":35,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,3]]}},"alternative-id":["10.1111\/sji.12405"],"URL":"https:\/\/doi.org\/10.1111\/sji.12405","archive":["Portico"],"relation":{},"ISSN":["0300-9475","1365-3083"],"issn-type":[{"value":"0300-9475","type":"print"},{"value":"1365-3083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3]]},"assertion":[{"value":"2015-03-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-12-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-03-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}