{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T19:07:19Z","timestamp":1790536039777,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,4,24]],"date-time":"2017-04-24T00:00:00Z","timestamp":1492992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010270","name":"Capital Health Research and Development of special","doi-asserted-by":"crossref","award":["2014-4-2162"],"award-info":[{"award-number":["2014-4-2162"]}],"id":[{"id":"10.13039\/501100010270","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Collaborative Innovation Center of Infectious Disease","award":["3500-115215"],"award-info":[{"award-number":["3500-115215"]}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["2014CB744403"],"award-info":[{"award-number":["2014CB744403"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National 12.5 grant","award":["2013ZX10003003"],"award-info":[{"award-number":["2013ZX10003003"]}]},{"name":"Beijing Science and Technology Foundation of China","award":["Z151100004015104"],"award-info":[{"award-number":["Z151100004015104"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2017,5]]},"abstract":"Abstract<\/jats:title>Upregulation ofCD<\/jats:styled-content>137 on recently activatedCD<\/jats:styled-content>8+<\/jats:sup>T cells has been used to identify rare viral and tumour antigen\u2010specific T cells from the peripheral blood. We aimed to evaluate the accuracy ofCD<\/jats:styled-content>137 for identifyingMycobacterium tuberculosis<\/jats:italic>(Mtb)\u2010reactiveCD<\/jats:styled-content>4+<\/jats:sup>T cells in the peripheral blood of infected individuals by flow cytometry and to investigate the characteristics of theseCD<\/jats:styled-content>137+<\/jats:sup>CD<\/jats:styled-content>4+<\/jats:sup>T cells. We initially enrolled 31 active tuberculosis (TB<\/jats:styled-content>) patients, 31 individuals with latentTB<\/jats:styled-content>infection (LTBI<\/jats:styled-content>) and 25 healthy donors. The intracellularCD<\/jats:styled-content>137 and interferon\u2010\u03b3<\/jats:italic>(IFN<\/jats:styled-content>\u2010\u03b3<\/jats:italic>) production byCD<\/jats:styled-content>4+<\/jats:sup>T cells was simultaneously detected under unstimulated andCFP<\/jats:styled-content>10\u2010stimulated (culture filtrate protein 10, a Mtb\u2010specific antigen) conditions. In unstimulatedCD<\/jats:styled-content>4+<\/jats:sup>T cells, we found that theCD<\/jats:styled-content>137 expression in theTB<\/jats:styled-content>group was significantly higher than that in theLTBI<\/jats:styled-content>group. Stimulation withCFP<\/jats:styled-content>10 largely increased theCD<\/jats:styled-content>4+<\/jats:sup>T cellCD<\/jats:styled-content>137 expression in both theTB<\/jats:styled-content>andLTBI<\/jats:styled-content>groups. AfterCFP<\/jats:styled-content>10 stimulation, the frequency ofCD<\/jats:styled-content>137+<\/jats:sup>CD<\/jats:styled-content>4+<\/jats:sup>T cells was higher than that ofIFN<\/jats:styled-content>\u2010\u03b3<\/jats:italic>+<\/jats:sup>CD<\/jats:styled-content>4+<\/jats:sup>T cells in both theTB<\/jats:styled-content>andLTBI<\/jats:styled-content>groups. Most of theCFP<\/jats:styled-content>10\u2010activatedIFN<\/jats:styled-content>\u2010\u03b3<\/jats:italic>\u2010secreting cells wereCD<\/jats:styled-content>137\u2010positive, but only a small fraction of theCD<\/jats:styled-content>137\u2010positive cells expressedIFN<\/jats:styled-content>\u2010\u03b3<\/jats:italic>. An additional 20 patients withTB<\/jats:styled-content>were enrolled to characterize theCD<\/jats:styled-content>45RO<\/jats:styled-content>+<\/jats:sup>CCR<\/jats:styled-content>7+<\/jats:sup>,CD<\/jats:styled-content>45RO<\/jats:styled-content>+<\/jats:sup>CCR<\/jats:styled-content>7\u2212<\/jats:sup>andCD<\/jats:styled-content>45RO<\/jats:styled-content>\u2212<\/jats:sup>subsets in theCD<\/jats:styled-content>137+<\/jats:sup>CD<\/jats:styled-content>4+<\/jats:sup>T cell populations. The Mtb\u2010specificCD<\/jats:styled-content>137+<\/jats:sup>CD<\/jats:styled-content>4+<\/jats:sup>T cells were mainly identified as having an effector memory phenotype. In conclusion,CD<\/jats:styled-content>137 is a useful marker that can be used for identifying Mtb\u2010reactiveCD<\/jats:styled-content>4+<\/jats:sup>T cells by flow cytometry.<\/jats:p>","DOI":"10.1111\/sji.12541","type":"journal-article","created":{"date-parts":[[2017,2,21]],"date-time":"2017-02-21T15:44:51Z","timestamp":1487691891000},"page":"372-380","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["CD<\/scp>137 is a Useful Marker for IdentifyingCD<\/scp>4+<\/sup>T Cell Responses toMycobacterium tuberculosis<\/i>"],"prefix":"10.1111","volume":"85","author":[{"given":"Z.\u2010h.","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Central Laboratory Beijing Key Laboratory for Drug Resistance Tuberculosis Research Beijing Chest Hospital Capital Medical University and, Beijing Tuberculosis and Thoracic Tumor Research Institute Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.\u2010f.","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Pathology Capital Medical University Beijing Chao\u2010yang Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Central Laboratory Beijing Key Laboratory for Drug Resistance Tuberculosis Research Beijing Chest Hospital Capital Medical University and, Beijing Tuberculosis and Thoracic Tumor Research Institute Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Central Laboratory Beijing Key Laboratory for Drug Resistance Tuberculosis Research Beijing Chest Hospital Capital Medical University and, Beijing Tuberculosis and Thoracic Tumor Research Institute Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.\u2010j.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Central Laboratory Beijing Key Laboratory for Drug Resistance Tuberculosis Research Beijing Chest Hospital Capital Medical University and, Beijing Tuberculosis and Thoracic Tumor Research Institute Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.\u2010j.","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Central Laboratory Beijing Key Laboratory for Drug Resistance Tuberculosis Research Beijing Chest Hospital Capital Medical University and, Beijing Tuberculosis and Thoracic Tumor Research Institute Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.\u2010y.","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Central Laboratory Beijing Key Laboratory for Drug Resistance Tuberculosis Research Beijing Chest Hospital Capital Medical University and, Beijing Tuberculosis and Thoracic Tumor Research Institute Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.\u2010j.","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Central Laboratory Navy General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.\u2010l.","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of National Tuberculosis Reference Laboratory Chinese Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.\u2010t.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Central Laboratory Beijing Key Laboratory for Drug Resistance Tuberculosis Research Beijing Chest Hospital Capital Medical University and, Beijing Tuberculosis and Thoracic Tumor Research Institute Beijing 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