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Then, several fusion proteins such as Rv2007c\u2010Rv2626c (F6), Rv2031c\u2010Rv1738\u2010Rv1733c (H83), <jats:styled-content style=\"fixed-case\">ESAT<\/jats:styled-content>6\u2010Rv1738\u2010Rv2626c (<jats:styled-content style=\"fixed-case\">LT<\/jats:styled-content>40), <jats:styled-content style=\"fixed-case\">ESAT<\/jats:styled-content>6\u2010Ag85B\u2010<jats:styled-content style=\"fixed-case\">MPT<\/jats:styled-content>64<jats:sub>&lt;190\u2010198&gt;<\/jats:sub>\u2010Mtb8.4 (<jats:styled-content style=\"fixed-case\">EAMM<\/jats:styled-content>), and <jats:styled-content style=\"fixed-case\">EAMM<\/jats:styled-content>\u2010Rv2626c (<jats:styled-content style=\"fixed-case\">LT<\/jats:styled-content>70) were constructed and their therapeutic effects were evaluated in pulmonary <jats:italic>Mycobacterium bovis<\/jats:italic> Bacilli Calmette\u2013Gu\u00e9rin (<jats:styled-content style=\"fixed-case\">BCG<\/jats:styled-content>) \u2013 latently infected rabbit or mouse models. The results showed that <jats:styled-content style=\"fixed-case\">EAMM<\/jats:styled-content> and F6 plus H83 had therapeutic effect against <jats:styled-content style=\"fixed-case\">BCG<\/jats:styled-content> latent infection in the rabbit model, respectively, and that the combination of <jats:styled-content style=\"fixed-case\">EAMM<\/jats:styled-content> with F6 plus H83 significantly reduced the bacterial load. In addition, the fusion proteins <jats:styled-content style=\"fixed-case\">LT<\/jats:styled-content>40 and <jats:styled-content style=\"fixed-case\">LT<\/jats:styled-content>70 consisting of multistage antigens showed promising therapeutic effects in the mouse model. We conclude that subunit vaccines consisting of both latency and replicating\u2010associated antigens show promising therapeutic effects in <jats:styled-content style=\"fixed-case\">BCG<\/jats:styled-content> latent infection animal models.<\/jats:p>","DOI":"10.1111\/sji.12556","type":"journal-article","created":{"date-parts":[[2017,4,20]],"date-time":"2017-04-20T13:59:11Z","timestamp":1492696751000},"page":"425-432","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Subunit Vaccines Consisting of Antigens from Dormant and Replicating Bacteria Show Promising Therapeutic Effect against <i>Mycobacterium Bovis <\/i><scp>BCG<\/scp> Latent Infection"],"prefix":"10.1111","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8422-4594","authenticated-orcid":false,"given":"F.","family":"Li","sequence":"first","affiliation":[{"name":"Gansu Key Lab of Evidence Based Medicine and Clinical Transfer Medicine &amp; Lanzhou Center for Tuberculosis Research School of Basic Medical Sciences Lanzhou University Lanzhou China"},{"name":"Institute of Pathogen Biology School of Basic Medical Sciences Lanzhou University  Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Kang","sequence":"additional","affiliation":[{"name":"Gansu Key Lab of Evidence Based Medicine and Clinical Transfer Medicine &amp; Lanzhou Center for Tuberculosis Research School of Basic Medical Sciences Lanzhou University Lanzhou China"},{"name":"Institute of Pathogen Biology School of Basic Medical Sciences Lanzhou University  Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Li","sequence":"additional","affiliation":[{"name":"Gansu Key Lab of Evidence Based Medicine and Clinical Transfer Medicine &amp; Lanzhou Center for Tuberculosis Research School of Basic Medical Sciences Lanzhou University Lanzhou China"},{"name":"Department of Immunology School of Basic Medical Sciences Lanzhou University Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Pathology School of Basic Medical Sciences Lanzhou University Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology Johns Hopkins Bloomberg School of Public Health Baltimore MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"A. 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