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Ad\u2010<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s were isolated and cultured <jats:italic>in vitro<\/jats:italic> and transfected with a lentivirus vector for overexpression of the therapeutic murine <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201035 gene. <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201035 expression in transfected <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201035\u2010<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s) was quantified by enzyme\u2010linked immunosorbent assay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>). The lymphocytes subsets after one\u2010way mixed lymphocyte culture and <jats:italic>in vivo<\/jats:italic> intravenous transplantation were analysed by flow cytometry to evaluate the immunosuppressive effects of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201035\u2010<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s. <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> was performed to examine <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201035 expression in lymphocyte culture. Mouse Ad\u2010<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s were isolated and cultured. <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201035 was expressed in the <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content> supernatant and serum after <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201035 transduction into Ad\u2010<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s by lentiviral vector transfection <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>. The percentage of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>25<jats:sup>+<\/jats:sup> T regulatory (Treg) cells in mice treated with <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201035\u2010<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s significantly increased. <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201035\u2010<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s upregulated the <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>25<jats:sup>+<\/jats:sup> Treg cells in the allogeneic mixed lymphocyte reaction system, and lowered the percentage of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T cells compared with the other two control groups (<jats:italic>P<\/jats:italic> &lt; 0.01). <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A expression significantly decreased and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 expression significantly increased in <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201035\u2010<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s when compared by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> to the control groups (<jats:italic>P<\/jats:italic> &lt; 0.01). <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201035\u2010transduced Ad\u2010<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s <jats:italic>in vivo<\/jats:italic> can enhance proliferation of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>25<jats:sup>+<\/jats:sup> Treg cells and suppress the function of effector T cells such as T helper (Th) 1, Th2 and Th17 cells and may reduce the development of allograft rejection. Our data suggest that transduced Ad\u2010<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s overexpressing <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201035 may provide a useful approach for basic research on cell\u2010based immunotolerance therapy for inducing transplantation tolerance.<\/jats:p>","DOI":"10.1111\/sji.12613","type":"journal-article","created":{"date-parts":[[2017,9,9]],"date-time":"2017-09-09T14:26:38Z","timestamp":1504967198000},"page":"389-395","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Mesenchymal Stem Cells Overexpressing Interleukin\u201035 Propagate Immunosuppressive Effects in Mice"],"prefix":"10.1111","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9094-9014","authenticated-orcid":false,"given":"H.","family":"Guo","sequence":"first","affiliation":[{"name":"Department of General Surgery Tianjin Medical University General 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