{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T02:12:54Z","timestamp":1788487974785,"version":"build-2803163510"},"reference-count":65,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,10,22]],"date-time":"2017-10-22T00:00:00Z","timestamp":1508630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004878","name":"Funda\u00e7\u00e3o de Ensino e Pesquisa de Uberaba","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004878","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The development of new biomarkers for the diagnosis and prognosis of ovarian cancer may provide an opportunity for new therapies. In this study, we aimed to compare cytokines (interleukin [<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>]\u20102,<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20105,<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106,<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108,<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 and tumour necrosis factor [<jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>]\u2010<jats:italic>\u03b1<\/jats:italic>) and nitric oxide (<jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>) metabolite levels in non\u2010neoplastic tumours, benign primary ovarian tumours and malignant primary ovarian neoplasms. The secondary aim was to relate cytokine and intracystic<jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>metabolite levels to clinical, laboratory and pathologic characteristics for patients with primary ovarian malignancies. We evaluated 110 patients with adnexal masses. Cytokine concentrations were quantified by enzyme\u2010linked immunosorbent assay and nitrate concentrations by enzymatic reduction of nitrite by nitrate reductase. Patients with malignant neoplasms had higher<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106,<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108 and<jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>levels compared to patients with benign neoplasms. Histologic grade 1 tumours were associated with elevated<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102 levels, whereas anaemia was associated with elevated<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 levels. On average, those patients with elevated<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108 levels also had a neutrophil\/lymphocyte ratio (<jats:styled-content style=\"fixed-case\">NLR<\/jats:styled-content>) greater than 2.6 and less than 36 months of disease\u2010free survival (DFS). Patients with normal<jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content>19\u20109 levels had elevated<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 levels.<jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010<jats:italic>\u03b1<\/jats:italic>was elevated in patients with two carcinogenesis and those with a platelet\/lymphocyte ratio (<jats:styled-content style=\"fixed-case\">PLR<\/jats:styled-content>) less than 300.<jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>levels were higher in patients with an<jats:styled-content style=\"fixed-case\">NLR<\/jats:styled-content>less than 2.6 and<jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content>19\u20109 greater than 35 U\/ml. Elevated intracystic cytokine levels, especially<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 and<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108, are associated with worse prognosis in ovarian cancer.<\/jats:p>","DOI":"10.1111\/sji.12617","type":"journal-article","created":{"date-parts":[[2017,9,28]],"date-time":"2017-09-28T16:19:03Z","timestamp":1506615543000},"page":"462-470","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Role of Intracystic Cytokines and Nitric Oxide in Ovarian Neoplasms"],"prefix":"10.1111","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2519-5583","authenticated-orcid":false,"given":"A.","family":"Martins\u2010Filho","sequence":"first","affiliation":[{"name":"Research Institute of Oncology (IPON)\/Discipline of Gynecology and Obstetrics Federal University of Tri\u00e2ngulo Mineiro Uberaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. P.","family":"Jammal","sequence":"additional","affiliation":[{"name":"Research Institute of Oncology (IPON)\/Discipline of Gynecology and Obstetrics Federal University of Tri\u00e2ngulo Mineiro Uberaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. C.","family":"Micheli","sequence":"additional","affiliation":[{"name":"Discipline of Pharmacology Federal University of Tri\u00e2ngulo Mineiro Uberaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. M.","family":"Tavares\u2010Murta","sequence":"additional","affiliation":[{"name":"Discipline of Pharmacology Federal University of Tri\u00e2ngulo Mineiro Uberaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. M.","family":"Etchebehere","sequence":"additional","affiliation":[{"name":"Surgical Pathology Service Federal University of Tri\u00e2ngulo Mineiro Uberaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. F. C.","family":"Murta","sequence":"additional","affiliation":[{"name":"Research Institute of Oncology (IPON)\/Discipline of Gynecology and Obstetrics Federal University of Tri\u00e2ngulo Mineiro Uberaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. S.","family":"Nomelini","sequence":"additional","affiliation":[{"name":"Research Institute of Oncology (IPON)\/Discipline of Gynecology and Obstetrics Federal University of Tri\u00e2ngulo Mineiro Uberaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,22]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"American Cancer Society.Cancer facts & figures 2013. Atlanta GA 2017. Available from:http:\/\/www.cancer.org\/Research\/CancerFactsFigures\/CancerFactsFigures\/ACSPC-036845 accessed on May 04 2017."},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21387"},{"key":"e_1_2_8_4_1","unstructured":"FerlayJ SoerjomataramI ErvikMet al.GLOBOCAN 2012 v1.0 Cancer Incidence and Mortality Worldwide: IARC Cancer Base No. 11[Internet].Lyon France:International Agency for Research on Cancer;2013. Available from:http:\/\/globocan.iarc.fr accessed on 04\/maio\/2017."},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098-2388(200007\/08)19:1<11::AID-SSU3>3.0.CO;2-3"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-008-0516-3"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.17772\/gp\/1579"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01307186"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07205"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)04046-0"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/82_2010_46"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2007.00869.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0031-6997(25)06663-3","article-title":"Nitric oxide: physiology, pathophysiology, and pharmacology","volume":"43","author":"Moncada S","year":"1991","journal-title":"Pharmacol Rev"},{"key":"e_1_2_8_14_1","first-page":"419","article-title":"Inducible nitric oxide synthase (iNOS) is expressed similarly in multiple aberrant crypt foci and colorectal tumors from the same patients","volume":"61","author":"Hao XP","year":"2001","journal-title":"Cancer Res"},{"key":"e_1_2_8_15_1","first-page":"707","article-title":"Ultrasonographic criteria and tumor marker assay are good procedures for the diagnosis of ovarian neoplasia in preselected outpatients","volume":"25","author":"Murta EFC","year":"2004","journal-title":"Eur J Gynaecol Oncol"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.gco.0000192967.67567.e9"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-014-3364-8"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3181cf3d79"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2011.03.003"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.7314\/APJCP.2016.17.3.1445"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12094-011-0687-9"},{"issue":"5","key":"e_1_2_8_22_1","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1177\/030089160409000509","article-title":"Differential tumor 9 microenvironment in human ovarian cystic tumors","volume":"90","author":"Tavares\u2010Murta BM","year":"2004","journal-title":"Tumori"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1086\/315682"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.12.7764"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12307-012-0124-9"},{"issue":"2","key":"e_1_2_8_26_1","first-page":"577","article-title":"Expression of cytokines interleukin\u20102, interleukin\u20104, interleukin\u201010 and transforming growth factor \u03b2 in gastric adenocarcinoma biopsies obtained from Mexican patients","volume":"18","author":"Diaz Orea MA","year":"2017","journal-title":"Asian Pac J Cancer Prev"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2011.08.003"},{"issue":"4","key":"e_1_2_8_28_1","first-page":"2509","article-title":"Prognostic relevance of soluble interleukin\u20102 receptors in patients with ovarian tumors","volume":"19","author":"Gebauer G","year":"1999","journal-title":"Anticancer Res"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/IGC.0b013e3182289ab1"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2009.03.028"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-1496"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-11-2178"},{"issue":"5","key":"e_1_2_8_33_1","first-page":"566","article-title":"Profiling of cytokines in human epithelial ovarian cancer ascites","volume":"2","author":"Matte I","year":"2012","journal-title":"Am J Cancer Res"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13048-015-0183-x"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19950215)75:4<1004::AID-CNCR2820750416>3.0.CO;2-G"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-0861"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000006752"},{"key":"e_1_2_8_38_1","first-page":"947831","article-title":"Parameters of blood count and tumor markers in patients with borderline ovarian tumors: a retrospective analysis and relation to staging","volume":"2012","author":"Nomelini RS","year":"2012","journal-title":"ISRN Oncol"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-364935-5.50008-0"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-6101(01)00016-8"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2012.04.013"},{"issue":"16","key":"e_1_2_8_42_1","first-page":"4610","article-title":"Acidic pH\u2010induced elevation in interleukin 8 expression by human ovarian carcinoma cells","volume":"60","author":"Xu L","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(01)00213-5"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-05-0326"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2014.07.254"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-012-2460-5"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-10-283"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2015.64"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11596-011-0161-5"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-08-0351"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(01)01804-0"},{"issue":"3","key":"e_1_2_8_52_1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1684\/ecn.2009.0161","article-title":"Circulating levels of TNF\u2010\u03b1 and its soluble receptors in the plasma of patients with epithelial ovarian cancer","volume":"20","author":"Dobrzycka B","year":"2009","journal-title":"Eur Cytokine Netw"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI39065"},{"issue":"3","key":"e_1_2_8_54_1","first-page":"375","article-title":"Proinflammatory and immunosuppressive serum, ascites and cyst fluid cytokines in patients with early and advanced ovarian cancer and benign ovarian tumors","volume":"31","author":"Nowak M","year":"2010","journal-title":"Neuro Endocrinol Lett"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2004.04.004"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aaf8608"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1910"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2014.07.014"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgt034"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2008.03.020"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2008.04.006"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0705358"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-1269"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005956721457"},{"key":"e_1_2_8_65_1","first-page":"655","article-title":"Studies on CA125, CA19.9, TPA and IAP as tumor markers in patients with ovarian cancer","volume":"6","author":"Negishi Y","year":"1985","journal-title":"Gan No Rinsho"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.12.4.16908"}],"container-title":["Scandinavian Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fsji.12617","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/sji.12617","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T22:50:26Z","timestamp":1750891826000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/sji.12617"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,22]]},"references-count":65,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1111\/sji.12617"],"URL":"https:\/\/doi.org\/10.1111\/sji.12617","archive":["Portico"],"relation":{},"ISSN":["0300-9475","1365-3083"],"issn-type":[{"value":"0300-9475","type":"print"},{"value":"1365-3083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,10,22]]},"assertion":[{"value":"2017-07-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-09-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}