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The purpose of this study was to quantify different leucocyte populations in peripheral blood of patients with p<jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>. Numbers of specific leucocyte subsets were determined in 86 p<jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content> patients and 74 healthy donors quantifying 21 distinct subtypes by flow cytometry. Subgroups of <jats:styled-content style=\"fixed-case\">pSS<\/jats:styled-content> patients were stratified based on presence of extraglandular manifestations (<jats:styled-content style=\"fixed-case\">EGM<\/jats:styled-content>s) and <jats:styled-content style=\"fixed-case\">SSA<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">SSB<\/jats:styled-content> autoantibodies. Overall, p<jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content> patients manifested decreased lymphocyte subpopulations compared to healthy donors. Such decreases were more pronounced in SSA\/<jats:styled-content style=\"fixed-case\">SSB<\/jats:styled-content> positive patients and patients with <jats:styled-content style=\"fixed-case\">EGM<\/jats:styled-content>. Granulocyte and monocyte subpopulations were increased in <jats:styled-content style=\"fixed-case\">pSS<\/jats:styled-content> patients compared to healthy donors, with the greatest increases in <jats:styled-content style=\"fixed-case\">SSA<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">SSB<\/jats:styled-content> positive patients. Unsupervised hierarchal clustering based on cell quantities was used to further subgroup the <jats:styled-content style=\"fixed-case\">pSS<\/jats:styled-content> patients into four clusters. One of the clusters characterized by higher concentrations of <jats:styled-content style=\"fixed-case\">NKT<\/jats:styled-content> cells, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>56hi <jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content> cells, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>20<jats:sup>+<\/jats:sup><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>38<jats:sup>\u2212<\/jats:sup> B cells and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>38<jats:sup>\u2212<\/jats:sup> T cells was associated with weaker clinical symptoms than the other clusters, possibly marking a milder disease phenotype. In conclusion, our analyses indicate significant alterations in the cellular profiles of peripheral blood leucocytes in patients with <jats:styled-content style=\"fixed-case\">pSS<\/jats:styled-content> and may help to stratify the patients according to disease severity.<\/jats:p>","DOI":"10.1111\/sji.12622","type":"journal-article","created":{"date-parts":[[2017,10,26]],"date-time":"2017-10-26T09:36:38Z","timestamp":1509010598000},"page":"491-502","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Patients with Primary Sj\u00f6gren's Syndrome Have Alterations in Absolute Quantities of Specific Peripheral Leucocyte Populations"],"prefix":"10.1111","volume":"86","author":[{"given":"R.","family":"Davies","sequence":"first","affiliation":[{"name":"Broegelmann Research Laboratory Department of Clinical Science University of Bergen Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Hammenfors","sequence":"additional","affiliation":[{"name":"Broegelmann Research Laboratory Department of Clinical Science University of Bergen Bergen Norway"},{"name":"Department of Rheumatology Haukeland University Hospital Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Bergum","sequence":"additional","affiliation":[{"name":"Broegelmann Research Laboratory Department of Clinical Science University of Bergen Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Jakobsen","sequence":"additional","affiliation":[{"name":"Broegelmann Research Laboratory Department of Clinical Science University of Bergen Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Solheim","sequence":"additional","affiliation":[{"name":"Department of Clinical Science University of Bergen Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Vogelsang","sequence":"additional","affiliation":[{"name":"Broegelmann Research Laboratory Department of Clinical Science University of Bergen Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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