{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:40:18Z","timestamp":1760985618485,"version":"3.37.3"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,11,26]],"date-time":"2017-11-26T00:00:00Z","timestamp":1511654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A glycoconjugate (dLPS\u2010CBSA) from hydrazine\u2010detoxified lipopolysaccharide (LPS) of <jats:italic>Vibrio cholerae<\/jats:italic> O139 and BSA was used for immunization of BALB\/c mice. The immunogenicity (concentration of induced IgG antibodies) of the conjugate was determined by enzyme\u2010linked immunosorbent assay. The highest level of LPS\u2010specific IgGs was found in sera collected 2\u00a0weeks after the third immunization. Both IgM and IgG antibodies present in the anti\u2010<jats:italic>V.\u00a0cholerae<\/jats:italic> O139\u2010specific sera showed long\u2010lasting vibriocidal activity. The glycoconjugate also stimulated production of IL\u20104, IL\u20106, IFN\u03b3, TNF\u03b1 and IL\u201010. Immunization with the conjugate induced significant production of both Th1 and Th2 cytokine sets, indicating balanced Th1\/Th2 immune response polarization. The quantitative analysis of total and LPS\u2010specific IgM and IgG antibodies production by B lymphocytes (from both the spleen and the bone marrow) was performed by ELISPOT assay. From the analysis of antibodies produced directly by B lymphocytes in the bone marrow (of dLPS\u2010CBSA immunized mice), we were able to identify the IgM\u2010IgG switch in the antibodies produced.<\/jats:p>","DOI":"10.1111\/sji.12623","type":"journal-article","created":{"date-parts":[[2017,10,24]],"date-time":"2017-10-24T08:13:29Z","timestamp":1508832809000},"page":"444-451","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Immunogenicity of a Glycoconjugate from Hydrazine\u2010Detoxified Lipopolysaccharide of <i>Vibrio cholerae<\/i> O139"],"prefix":"10.1111","volume":"86","author":[{"given":"A.","family":"Fleischhackerov\u00e1\u2010Slim\u00e1kov\u00e1","sequence":"first","affiliation":[{"name":"Department of Immunochemistry of Glycoconjugates Institute of Chemistry Slovak Academy of Sciences Bratislava Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8769-1825","authenticated-orcid":false,"given":"J.","family":"Korcov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Immunochemistry of Glycoconjugates Institute of Chemistry Slovak Academy of Sciences Bratislava Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Bystrick\u00fd","sequence":"additional","affiliation":[{"name":"Department of Immunochemistry of Glycoconjugates Institute of Chemistry Slovak Academy of Sciences Bratislava Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,11,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","unstructured":"WaldorMK MekalanosJJ.Progress toward Live\u2010Attenuated Cholera Vaccines. In:Kryono H Ogra PL McGhee JR eds. Mucosal Vaccines.San Diego:Academic Press 1996:229\u201340.","DOI":"10.1016\/B978-012410580-5\/50018-2"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.24.11388"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(95)90698-3"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(95)90667-3"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007164216202"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.9.5037-5043.2000"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.5.3488-3493.2001"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/09168451.2014.942251"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-030409-101159"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1517\/14740338.5.3.351"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00046-08"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.60.8.3201-3208.1992"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(91)90139-7"},{"key":"e_1_2_6_15_1","first-page":"1","article-title":"Conjugate vaccines","volume":"1191","author":"Goldblatt D","year":"2000","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2006.03077.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.020875-0"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.2478\/s11756-010-0092-9"},{"key":"e_1_2_6_19_1","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1128\/cdli.2.6.685-688.1995","article-title":"Comparison of the vibriocidal antibody response in cholera due to Vibrio cholerae O139 Bengal with the response in cholera due to Vibrio cholerae O1","volume":"2","author":"Quadri F","year":"1995","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2006.tb03835.x"},{"key":"e_1_2_6_21_1","first-page":"15","article-title":"Handbook of ELISPOT: Methods and protocols. Totowa, NJ: Humana Press Inc","volume":"302","author":"Kalyuzhny AE","year":"2005","journal-title":"Methods Mol Biol"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000221"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/121.5.505"},{"key":"e_1_2_6_24_1","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/iai.64.1.10-15.1996","article-title":"Factors influencing secondary vibriocidal immune responses: relevance for understanding immunity to cholera","volume":"64","author":"Losonsky GA","year":"1996","journal-title":"Inf Immun"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1086\/421275"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2012.07.004"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2004.08.013"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00192-08"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.294.23.3019"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00037-12"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(98)80162-3"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80541-5"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.3.1895-1902.1995"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-5890(01)00025-6"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e3181783adf"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00868-09"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.13.040195.003255"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2009.11.002"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201606116"}],"container-title":["Scandinavian Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fsji.12623","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/sji.12623","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T14:16:51Z","timestamp":1696688211000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/sji.12623"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,26]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1111\/sji.12623"],"URL":"https:\/\/doi.org\/10.1111\/sji.12623","archive":["Portico"],"relation":{},"ISSN":["0300-9475","1365-3083"],"issn-type":[{"type":"print","value":"0300-9475"},{"type":"electronic","value":"1365-3083"}],"subject":[],"published":{"date-parts":[[2017,11,26]]},"assertion":[{"value":"2017-03-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-11-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}