{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T11:39:19Z","timestamp":1793014759025,"version":"4.5.0"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,3,5]],"date-time":"2018-03-05T00:00:00Z","timestamp":1520208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["810470946"],"award-info":[{"award-number":["810470946"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Studies show that the Th17\/<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A axis plays an important role in the pathogenesis of kidney diseases. Previously, we also showed that <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A may play a role in the pathogenesis of primary nephrotic syndrome; however, the underlying mechanism(s) is unclear. The aim of this study was to explore the molecular mechanism of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A\u2010inducing podocyte injury in\u00a0vitro. In this study, the <jats:styled-content style=\"fixed-case\">NLRP<\/jats:styled-content>3 inflammasome activation and the morphology of podocytes were detected by Western blot and immunofluorescence. The results showed that podocytes persistently expressed <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A receptor and that <jats:styled-content style=\"fixed-case\">NLRP<\/jats:styled-content>3 inflammasome in these cells was activated upon exposure to <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A. Also, activity of caspase\u20101 and secretion of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2 increased in the presence of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A. In addition, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A disrupted podocyte morphology by decreasing expression of podocin and increasing expression of desmin. Blockade of intracellular <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> or inhibition of caspase\u20101 prevented activation of the <jats:styled-content style=\"fixed-case\">NLRP<\/jats:styled-content>3 inflammasome, thereby restoring podocyte morphology. Taken together, the results suggest that <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A induces podocyte injury by activating the <jats:styled-content style=\"fixed-case\">NLRP<\/jats:styled-content>3 inflammasome and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2 secretion and contributes to disruption of the kidney's filtration system.<\/jats:p>","DOI":"10.1111\/sji.12645","type":"journal-article","created":{"date-parts":[[2018,2,15]],"date-time":"2018-02-15T10:42:44Z","timestamp":1518691364000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Interleukin\u201017A participates in podocyte injury by inducing <scp>IL<\/scp>\u20101\u03b2 secretion through <scp>ROS<\/scp>\u2010<scp>NLRP<\/scp>3 inflammasome\u2010caspase\u20101 pathway"],"prefix":"10.1111","volume":"87","author":[{"given":"J.","family":"Yan","sequence":"first","affiliation":[{"name":"Key Laboratory of the Ministry of Education Key Laboratory of Pediatrics in Chongqing Chongqing International Science and Technology Cooperation Center for Child Development and Disorders Chongqing Key Laboratory of Child Infection and Immunity Children's Hospital of Chongqing Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of the Ministry of Education Key Laboratory of Pediatrics in Chongqing Chongqing International Science and Technology Cooperation Center for Child Development and Disorders Chongqing Key Laboratory of Child Infection and Immunity Children's Hospital of Chongqing Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4245-9376","authenticated-orcid":false,"given":"H.","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Nephrology Children's Hospital of Chongqing Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of the Ministry of Education Key Laboratory of Pediatrics in Chongqing Chongqing International Science and Technology Cooperation Center for Child Development and Disorders Chongqing Key Laboratory of Child Infection and Immunity Children's Hospital of Chongqing Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Nephrology Children's Hospital of Chongqing Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Nephrology Children's Hospital of Chongqing Medical University Chongqing 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