{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:23:12Z","timestamp":1759332192852,"version":"3.41.2"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,4,16]],"date-time":"2018-04-16T00:00:00Z","timestamp":1523836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006048","name":"Universidad de la Rep\u00fablica Uruguay","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006048","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006048","name":"Universidad de la Rep\u00fablica Uruguay","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006048","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tissue transglutaminase is a ubiquitous and multifunctional protein that contributes to several processes such as apoptosis\/survival, efferocytosis, inflammation and tissue repairing under physiological and pathological conditions. Several activities can be associated with well\u2010established functional domains; in addition, four <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> alternative splice variants have been described, characterized by sequence divergences and residues deletion at the C\u2010terminal domains. Tissue transglutaminase is recognized as the central player in the physiopathology of coeliac disease (CD) mainly through calcium\u2010dependent enzymatic activities. It can be hypothesized that differential regulation of tissue transglutaminase splice variants expression in persons with CD contributes to pathology by altering the protein functionality. We characterized the expression pattern of <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> alternative splice variants by <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> in peripheral cells from patients with CD under free gluten diet adhesion; we considered inflammatory parameters and specific antibodies as markers of the stage of disease. We found significant higher expression of both the full length and the shortest C\u2010truncated splice variants in leucocytes from patients with CD in comparison with healthy individuals. As tissue transglutaminase expression and canonical enzymatic activity are linked to inflammation, we studied the <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression of inflammatory cytokines in peripheral leucocytes of persons with CD in relation with splice variants expression; interestingly, we found that recently diagnosed patients showed significant correlation between both the full length and the shortest alternative spliced variants with <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101 expression. Our results points that regulation of alternative splicing of tissue transglutaminase could account for the complex physiopathology of CD.<\/jats:p>","DOI":"10.1111\/sji.12659","type":"journal-article","created":{"date-parts":[[2018,3,15]],"date-time":"2018-03-15T15:04:48Z","timestamp":1521126288000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Alternative <scp>RNA<\/scp> splicing of leucocyte tissue transglutaminase in coeliac disease"],"prefix":"10.1111","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9206-8667","authenticated-orcid":false,"given":"P.","family":"Arbildi","sequence":"first","affiliation":[{"name":"C\u00e1tedra de Inmunolog\u00eda Facultad de Qu\u00edmica y Facultad de Ciencias Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2993-7513","authenticated-orcid":false,"given":"C.","family":"S\u00f3\u00f1ora","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Inmunolog\u00eda Facultad de Qu\u00edmica y Facultad de Ciencias Universidad de la Rep\u00fablica Montevideo Uruguay"},{"name":"Facultad de Medicina Escuela Universitaria de Tecnolog\u00eda M\u00e9dica Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Del R\u00edo","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Inmunolog\u00eda Facultad de Qu\u00edmica y Facultad de Ciencias Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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