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Although <jats:styled-content style=\"fixed-case\">ITP<\/jats:styled-content> was originally thought to be primarily due to antibody\u2010mediated autoimmunity, it is now clear that T cells also play a significant role in the disease. However, the exact interplay between platelet destruction, megakaryocyte dysfunction and the elements of both humoral and cell\u2010mediated immunity in <jats:styled-content style=\"fixed-case\">ITP<\/jats:styled-content> remains incompletely defined. While most studies have focused on immune platelet destruction in the spleen, an additional possibility is that the antiplatelet antibodies can also destroy bone marrow megakaryocytes. To address this, we negated the effects of T cells by utilizing an in\u00a0vivo passive <jats:styled-content style=\"fixed-case\">ITP<\/jats:styled-content> model where <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice were administered various anti\u2010\u03b1<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>b, anti\u2010\u03b23 or anti\u2010<jats:styled-content style=\"fixed-case\">GPI<\/jats:styled-content>b antibodies or antisera and platelet counts and bone marrow megakaryocytes were enumerated. Our results show that after 24\u00a0hours, all the different antiplatelet antibodies\/sera induced variable degrees of thrombocytopenia in recipient mice. Compared with na\u00efve control mice, however, histological examination of the bone marrow revealed that only 2 antibody preparations (mouse\u2010anti\u2010mouse \u03b23 sera and an anti\u2010 \u03b1<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>b monoclonal antibody (<jats:styled-content style=\"fixed-case\">MWR<\/jats:styled-content>eg30) could affect bone marrow megakaryocyte counts. Our study shows that while most antiplatelet antibodies induce acute thrombocytopenia, the majority of them do not affect the number of megakaryocytes in the bone marrow. This suggests that other mechanisms may be responsible for megakaryocyte abnormalities seen during immune thrombocytopenia.<\/jats:p>","DOI":"10.1111\/sji.12678","type":"journal-article","created":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T07:46:02Z","timestamp":1527839162000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Antiplatelet antibody\u2010induced thrombocytopenia does not correlate with megakaryocyte abnormalities in murine immune thrombocytopenia"],"prefix":"10.1111","volume":"88","author":[{"given":"L.","family":"Guo","sequence":"first","affiliation":[{"name":"The Toronto Platelet Immunobiology Group Toronto ON Canada"},{"name":"Keenan Research Centre for Biomedical Science of St. Michael's Hospital Toronto ON Canada"},{"name":"Institute of Medical Science University of Toronto Toronto ON Canada"},{"name":"University of Utah Salt Lake City UT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Kapur","sequence":"additional","affiliation":[{"name":"The Toronto Platelet Immunobiology Group Toronto ON Canada"},{"name":"Keenan Research Centre for Biomedical Science of St. Michael's Hospital Toronto ON Canada"},{"name":"Institute of Medical Science University of Toronto Toronto ON Canada"},{"name":"Canadian Blood Services Lund University Canadian Blood Services Toronto ON Canada"},{"name":"Division of Hematology and Transfusion Medicine Lund University Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Aslam","sequence":"additional","affiliation":[{"name":"The Toronto Platelet Immunobiology Group Toronto ON Canada"},{"name":"Keenan Research Centre for Biomedical Science of St. Michael's Hospital Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Hunt","sequence":"additional","affiliation":[{"name":"The Toronto Platelet Immunobiology Group Toronto ON Canada"},{"name":"Keenan Research Centre for Biomedical Science of St. Michael's Hospital Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Hou","sequence":"additional","affiliation":[{"name":"The Toronto Platelet Immunobiology Group Toronto ON Canada"},{"name":"Keenan Research Centre for Biomedical Science of St. Michael's Hospital Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Zufferey","sequence":"additional","affiliation":[{"name":"The Toronto Platelet Immunobiology Group Toronto ON Canada"},{"name":"Keenan Research Centre for Biomedical Science of St. Michael's Hospital Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. 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