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Tregs suppress immune responses and promote the development of tumours in patients with\u00a0EOC. However, the underlying mechanisms remain unclear. In this study, we found higher levels of CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>high<\/jats:sup>CD127<jats:sup>low<\/jats:sup> Tregs in\u00a0patients with EOC than in patients with benign ovarian tumours and healthy donors. The immune inhibitory effect of Tregs functions by maintaining high levels of immunosuppressive cytokines in EOC. The high levels of Tregs and related cytokines (TGF\u2010\u03b21 or IL\u201010) were associated with lymphatic metastasis and FIGO stages of patients with EOC. Expression of matrix metalloproteinase (MMP)\u20102 and tissue inhibitors of metalloproteinase (TIMP)\u20102 in EOC cell lines were significantly regulated in the coculture experiment with CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>high<\/jats:sup>CD127<jats:sup>low <\/jats:sup>Tregs sorted from EOC patients. Levels of MMP\u20102 and TIMP\u20102 conversely changed after blocking IL\u201010R and TGF\u2010\u03b21R in EOC cells. The invasion ability of EOC cells was also significantly downregulated in this process. The metastasis of EOC cells was correlated with the levels of TGF\u2010\u03b21 or IL\u201010. These findings suggested that immunosuppressive cytokines secreted by CD4<jats:sup>+<\/jats:sup> Tregs could be a novel target for inhibiting EOC progression.<\/jats:p>","DOI":"10.1111\/sji.12736","type":"journal-article","created":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T22:20:56Z","timestamp":1543443656000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Targeting cytokines secreted by CD4<sup>+<\/sup>CD25<sup>high<\/sup>CD127<sup>low<\/sup>\u00a0regulatory T cells inhibits ovarian cancer progression"],"prefix":"10.1111","volume":"89","author":[{"given":"Xing","family":"Ke","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Xinhua Hospital, Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6647-4749","authenticated-orcid":false,"given":"Lisong","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Xinhua Hospital, Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,12,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00188.2015"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(20001115)89:10<2068::AID-CNCR6>3.0.CO;2-Z"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.19216"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCO.0000000000000111"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-016-1825-6"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00620"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-015-1753-x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aak9702"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00487"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-014-1632-x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.4161\/epi.26675"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13048-017-0338-z"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2010.02.001"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1203224"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2017.6707"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0094476"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1590\/1414-431x20176104"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20170973"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-10-185"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00051"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-17-1365"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.31115"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2720"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2016.10.001"}],"container-title":["Scandinavian Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fsji.12736","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/sji.12736","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T06:01:06Z","timestamp":1694671266000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/sji.12736"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,27]]},"references-count":24,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["10.1111\/sji.12736"],"URL":"https:\/\/doi.org\/10.1111\/sji.12736","archive":["Portico"],"relation":{},"ISSN":["0300-9475","1365-3083"],"issn-type":[{"value":"0300-9475","type":"print"},{"value":"1365-3083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,27]]},"assertion":[{"value":"2018-08-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12736"}}