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In this model, during the first month there is a temporal control of bacillary growth, in coexistence with macrophage activation, granuloma formation and Th\u20101 response. Then, bacterial proliferation recommences, accompanied by progressive pneumonia and decreasing expression of protective cytokines (IFN\u2010\u03b3 and TNF\u2010\u03b1). In this model, we studied the IL\u201012 gene expression kinetics and cellular source. There is a rapid and progressive IL\u201012 expression peaking at day 14, when granulomas start their formation and numerous macrophages show strong IL\u201012 immunostaining, while during progressive TB there is a significant decrease of IL\u201012 expression and occasional macrophages showed IL\u201012 immunolabeling. In the second part of this study, we determined the immunotherapeutic effect of recombinant adenoviruses that codify IL\u201012 (AdIL\u201012). Intratracheal administration of only one dose of AdIL\u201012 one day before Mtb infection produced significant decrease of bacterial loads, lesser pneumonia and higher expression of TNF\u2010\u03b1, IFN\u2010\u03b3 and iNOS. When only one dose of AdIL\u201012 was given in healthy mice cohoused with infected mice with highly virulent and transmissible Mtb, total prevention of infection was conferred. Moreover, when AdIL\u201012 was administered by intranasal route in animals suffering late active TB after 2\u00a0months of infection, a very low pulmonary bacilli burdens was detected. These experimental data confirm that IL\u201012 is a significant cytokine in the immune protection against Mtb, and gene therapy based in adenoviruses coding this cytokine increased protective immunity and prevent Mtb transmission.<\/jats:p>","DOI":"10.1111\/sji.12743","type":"journal-article","created":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T15:59:05Z","timestamp":1544716745000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Immunotherapeutic effects of recombinant adenovirus encoding interleukin 12 in experimental pulmonary tuberculosis"],"prefix":"10.1111","volume":"89","author":[{"given":"Dulce Adriana","family":"Mata\u2010Espinosa","sequence":"first","affiliation":[{"name":"Secci\u00f3n de Patolog\u00eda Experimental Departamento de Patolog\u00eda Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubiran Mexico City 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