{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:09:00Z","timestamp":1785434940026,"version":"3.56.0"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,5,9]],"date-time":"2019-05-09T00:00:00Z","timestamp":1557360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Acinetobacter baumannii<\/jats:italic> (<jats:italic>A baumannii<\/jats:italic>) is an emerging nosocomial pathogenic bacterium which leads to hospital infections. The increase in drug\u2010resistant <jats:italic>A baumannii<\/jats:italic> strains makes it difficult to control by using common antibiotics. The development of effective vaccines is an alternative means to avoid <jats:italic>A baumannii<\/jats:italic> infections. In the present study, Balb\/c mice were inoculated intratracheally with 30\u00a0\u03bcg of OmpK\/Omp22 fusion protein alone or OmpK\/Omp22 formulated with MF59 adjuvant. After two times of boosting at day 14 and 21, the antigen\u2010specific antibody levels and the protective immunity against <jats:italic>A baumannii<\/jats:italic> challenge were evaluated. The results showed that the OmpK\/Omp22 formulated with MF59 immunized mice produced much higher level of antigen\u2010specific antibodies compared to mice immunized with OmpK\/Omp22 alone (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.01). Mice immunized with 30\u00a0\u03bcg of OmpK\/Omp22 formulated with MF59 also provided more potent protection post\u2010challenge, which showed lower bacterial loads in the blood and lung tissue, lower level of blood inflammatory cytokines and higher survival rate (83.3%) than mice immunized with OmpK\/Omp22 alone (<jats:italic>P\u00a0&lt;\u00a0<\/jats:italic>0.001). In conclusion, this study demonstrated that OmpK\/Omp22 fusion protein adjuvanted with MF59 induced superior immune response and better protection than OmpK\/Omp22 alone through intratracheal inoculation in mice<jats:bold>.<\/jats:bold><\/jats:p>","DOI":"10.1111\/sji.12769","type":"journal-article","created":{"date-parts":[[2019,4,21]],"date-time":"2019-04-21T13:49:35Z","timestamp":1555854575000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["MF59 adjuvant enhances the immunogenicity and protective immunity of the OmpK\/Omp22 fusion protein from <i>Acineterbacter baumannii<\/i> through intratracheal inoculation in mice"],"prefix":"10.1111","volume":"90","author":[{"given":"Ai\u2010qiong","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Pharmacy Affiliated Hospital of North Sichuan Medical College Nanchong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai\u2010yan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Affiliated Hospital of North Sichuan Medical College Nanchong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"San\u2010jun","family":"Guo","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Molecular Biology North Sichuan Medical College Nanchong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7061-1291","authenticated-orcid":false,"given":"Yong\u2010en","family":"Xie","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Molecular Biology North Sichuan Medical College Nanchong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1789"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2009.01820.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000089936.09573.F3"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamcollsurg.2010.12.024"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCC.0000000000000337"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077631"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00158"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep20724"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.10.064"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.03.031"},{"key":"e_1_2_7_12_1","first-page":"155","article-title":"Evaluation of the Protective efficacy of a fused OmpK\/Omp22 protein vaccine candidate against Acinetobacter baumannii infection in mice","volume":"31","author":"Guo SJ","year":"2018","journal-title":"Biomed Environ Sci"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01686-16"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.7883\/yoken.JJID.2015.560"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2016.04.019"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.02.083"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1101764"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(98)00252-7"},{"key":"e_1_2_7_19_1","first-page":"1","article-title":"Immunoprotective potential of in silico predicted Acinetobacter baumannii outer membrane nuclease, NucAb","volume":"306","author":"Garg N","year":"2016","journal-title":"Int J Microbiol"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.10.052"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.06.001"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00741-10"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.11.008"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077631"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trivac.2016.03.001"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389201014666131226123511"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2015.1052354"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17653-y"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01184-12"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2015.07.031"},{"key":"e_1_2_7_31_1","first-page":"1296","article-title":"Host Fate is Rapidly determined by innate effector\u2010microbial interactions during Acinetobacter baumannii bacteremia","volume":"211","author":"Bruhn KW","year":"2015","journal-title":"J Infect Dis"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00441"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2009.03893.x"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:PHAM.0000012160.00222.55"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0063344"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-010-0860-4"}],"container-title":["Scandinavian Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/sji.12769","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/sji.12769","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/sji.12769","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T18:13:25Z","timestamp":1694369605000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/sji.12769"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,9]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["10.1111\/sji.12769"],"URL":"https:\/\/doi.org\/10.1111\/sji.12769","archive":["Portico"],"relation":{},"ISSN":["0300-9475","1365-3083"],"issn-type":[{"value":"0300-9475","type":"print"},{"value":"1365-3083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,9]]},"assertion":[{"value":"2018-09-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12769"}}