{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T07:36:18Z","timestamp":1778657778632,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,6,7]],"date-time":"2019-06-07T00:00:00Z","timestamp":1559865600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31771010"],"award-info":[{"award-number":["31771010"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>TAM family members (TYRO3, AXL and MERTK) play essential roles in the resolution of inflammation and in infectious diseases and cancer. AXL, a tyrosine kinase receptor, is commonly overexpressed in several solid tumours and numerous hematopoietic malignancies including acute myeloid leukaemia, acute lymphocytic leukaemia, chronic myeloid leukaemia, chronic lymphocytic leukaemia and multiple myeloma. AXL significantly promotes tumour cell migration, invasion and metastasis, as well as angiogenesis. AXL also plays an important role in inflammation and macrophage ontogeny. Recent studies have revealed that AXL contributes to leukaemic phenotypes through activation of oncogenic signalling pathways that lead to increased cell migration and proliferation. To evaluate the mechanisms underlying the role of AXL signalling in tumour metastasis, we screened a phage display library to generate a novel human monoclonal antibody, named DAXL\u201088, that recognizes both human and murine AXL. The concentrations of DAXL\u201088 required for 50% maximal binding to human and murine AXL were 0.118 and 0.164\u00a0\u03bcg\/mL, respectively. Furthermore, DAXL\u201088 bound to human AXL with high affinity (<jats:italic>K<\/jats:italic><jats:sub>D<\/jats:sub>\u00a0~\u00a0370\u00a0pM). DAXL\u201088 blocked the interaction between AXL and its ligand, growth arrest\u2010specific gene 6 (GAS6), with a half maximal inhibitory concentration of 2.16\u00a0\u03bcg\/mL. Moreover, DAXL\u201088 inhibited AXL\/GAS6\u2010dependent cell signalling, which is implicated in cell migration and invasion. In conclusion, the novel anti\u2010AXL DAXL\u201088 high\u2010affinity antibody blocks the interaction between AXL and GAS6 and inhibits tumour cell migration and invasion induced by GAS6. Thus, DAXL\u201088 offers promise for the development of targeted therapeutic strategies in solid tumours, leukaemias and other lymphoid neoplasms.<\/jats:p>","DOI":"10.1111\/sji.12777","type":"journal-article","created":{"date-parts":[[2019,5,10]],"date-time":"2019-05-10T16:04:04Z","timestamp":1557504244000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["A novel human anti\u2010AXL monoclonal antibody attenuates tumour cell migration"],"prefix":"10.1111","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1572-002X","authenticated-orcid":false,"given":"Yanting","family":"Duan","sequence":"first","affiliation":[{"name":"State Key Laboratory of Toxicology and Medical Countermeasures Beijing Institute of Pharmacology and Toxicology Beijing China"},{"name":"Beijing Key Laboratory of Therapeutic Gene Engineering Antibody Beijing 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