{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T18:02:42Z","timestamp":1787767362423,"version":"build-2784847793"},"reference-count":60,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T00:00:00Z","timestamp":1561507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81500452"],"award-info":[{"award-number":["81500452"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The epoxyeicosatrienoic acids (EETs) are products of cytochrome P450 epoxygenases and have recently been found to have an anti\u2010inflammatory activity. However, the role of EETs in non\u2010alcoholic steatohepatitis has not been fully understood. In this study, we investigated the protective role of EETs in methionine\u2010choline\u2010deficient (MCD) diet\u2013induced non\u2010alcoholic steatohepatitis (NASH) in mice and the potential mechanisms. We used 1\u2010trifluoromethoxyphenyl\u20103\u2010(1\u2010propionylpiperidin\u20104\u2010yl)urea(TPPU), a soluble epoxide hydrolase inhibitor, to increase the endogenous EET level in mice. Upon TPPU treatment, the liver steatosis and inflammatory damage were significantly ameliorated in mice with steatohepatitis, paralleled by the downregulation of pro\u2010inflammatory cytokines (TNF\u2010\u03b1, IL\u20101\u03b2, IL\u20106) as well as chemokines (CXCL1, MCP\u20101). Compared with untreated NASH mice, mRNA levels of sterol regulatory element binding protein 1c (SREBP1c) and inflammation relevant adhesion molecules (ICAM\u20101, VCAM\u20101) were downregulated, whereas mRNA level of peroxisome proliferator\u2013activated receptor \u03b1(PPAR\u2010\u03b1) was elevated in TPPU\u2010treated mice. In vitro, 11,12\u2010EET treatment remarkably attenuated free fatty acid (FFA)\u2013induced inflammation in HepG2 and THP\u20101 cells. Further, 11,12\u2010EET inhibited the activation of NF\u2010\u03baB signalling pathway in macrophages from mice with steatohepatitis. Collectively, these results suggest that EETs play a protective role and alleviate the MCD diet\u2013induced steatohepatitis in mice mainly by downregulating activation of NF\u2010\u03baB pathway in macrophages.<\/jats:p>","DOI":"10.1111\/sji.12791","type":"journal-article","created":{"date-parts":[[2019,5,27]],"date-time":"2019-05-27T16:29:03Z","timestamp":1558974543000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Epoxyeicosatrienoic acids alleviate methionine\u2010choline\u2010deficient diet\u2013induced non\u2010alcoholic steatohepatitis in mice"],"prefix":"10.1111","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6181-0718","authenticated-orcid":false,"given":"Xiaojing","family":"Wang","sequence":"first","affiliation":[{"name":"Department and Institute of Infectious Disease Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan 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