{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T00:47:03Z","timestamp":1793234823291,"version":"4.5.0"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,10]],"date-time":"2019-11-10T00:00:00Z","timestamp":1573344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Sepsis is a life\u2010threatening disease that is an immune disorder response that causes multiple organ dysfunction. In this study, we investigated the dynamic changes in mRNA expression of HLA\u2010DRA gene and the specific transcription factor of helper T cell subsets to explore long\u2010term immunophenotyping and its relationship with prognosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Seventy\u2010eight sepsis patients and twelve healthy controls were recruited in this study. Blood samples were collected at eight\u2010time points during their septic course and were assayed for the gene expression of HLA\u2010DRA and T helper cell subset\u2010specific transcription factors (T\u2010bet: Th1, GATA3: Th2, Foxp3: Treg, RORC: Th17).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The levels of HLA\u2010DRA in survivors gradually increased but were maintained at lower levels in non\u2010survivors. The specific transcription factor of Th1 and Th2 cells, T\u2010bet and GATA\u20103 were significantly lower in sepsis patients than in normal controls, and the non\u2010survivors showed significantly lower levels than the survivors (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.05). RORC and FOXP3, the specific transcription factor of Treg and Th17 were significantly higher in survivors than in non\u2010survivors and normal controls (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.05). T\u2010bet and GATA\u20103 had a linear correlation with HLA\u2010DRA expression (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.01).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The dynamic changes in HLA\u2010DRA expression in peripheral blood could accurately reflect the immune status of sepsis patients, and the reduction in HLA\u2010DRA may be an important reason for abnormal T cell differentiation. The sustained low levels of the Th cell subsets (Th1 and Th2) suggest the suppression of adaptive immunity, and this persistent immunosuppression may be the leading cause of death in septic patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/sji.12813","type":"journal-article","created":{"date-parts":[[2019,8,6]],"date-time":"2019-08-06T09:08:25Z","timestamp":1565082505000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Dynamic changes in human HLA\u2010DRA gene expression and Th cell subsets in sepsis: Indications of immunosuppression and associated outcomes"],"prefix":"10.1111","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8608-8052","authenticated-orcid":false,"given":"Jianqiao","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine the Chinese PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Beijing Shijitan Hospital, Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine the Chinese PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sifan","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine the Chinese PLA General Hospital Beijing China"},{"name":"School of Medicine Nankai University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine the Chinese PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine the first affiliated hospital of the Chinese PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixin","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine the Chinese PLA General Hospital Beijing 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