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Macrophage polarization was characterized by measurement of molecular markers including Ym\u20101, Arg\u20101, TNF\u2010\u03b1 and iNOS via both real\u2010time PCR and Western blot. Vascular endothelial growth factor (VEGF) content was determined by enzyme\u2010linked immunosorbent assay. Choroidal neovascularization (CNV) formation was evaluated by choroidal flat mount technique. The regulatory action of miR\u2010505\u20105p on 3\u2032UTR of Transmembrane Protein 229B (TMEM229B) was interrogated by luciferase reporter assay. miR\u2010505 was aberrantly upregulated in both AMD and laser\u2010induced choroidal neovascularization mouse model. Administration with miR\u2010505 specific inhibitor suppressed M2 polarization in CNV mice as indicated by decreasing both Ym\u20101 and Arg\u20101. Meanwhile, VEGF expression and CNV formation were greatly suppressed by miR\u2010505 inhibition as well. The similar phenotype was consolidated in Prostaglandin E2 (PGE2)\u2010stimulated bone marrow\u2010derived macrophages. At the molecular level, miR\u2010505\u20105p directly targeted and negatively regulated TMEM229B expression, while forced ectopic expression of TMEM229B significantly rescued miR\u2010505\u2010imposed M2 polarization. Our data have uncovered the critical contribution of miR\u2010505 in AMD, which is predominantly mediated by downregulation of TMEM229B.<\/jats:p>","DOI":"10.1111\/sji.12832","type":"journal-article","created":{"date-parts":[[2019,9,23]],"date-time":"2019-09-23T06:28:24Z","timestamp":1569220104000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["MiR\u2010505 promotes M2 polarization in choroidal neovascularization model mice by targeting transmembrane protein 229B"],"prefix":"10.1111","volume":"90","author":[{"given":"Su","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Ophthalmology The Affiliated Hospital of Guizhou Medical University Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Lu","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Ophthalmology Shenzhen Eye Hospital Shenzhen 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