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The incidence of NT1 increased in Sweden, Finland and Norway following Pandemrix\u00ae\u2010vaccination, initiated to prevent the 2009 influenza pandemic. The pathogenesis of NT1 is poorly understood, and causal links to vaccination are yet to be clarified. The strong association with <jats:italic>Human leukocyte antigen<\/jats:italic> (<jats:italic>HLA<\/jats:italic>) <jats:italic>DQB1*06:02<\/jats:italic> suggests an autoimmune pathogenesis, but proposed autoantigens remain controversial. We used a two\u2010step approach to identify autoantigens in patients that acquired NT1 after Pandemrix\u00ae\u2010vaccination. Using arrays of more than 9000 full\u2010length human proteins, we screened the sera of 10 patients and 24 healthy subjects for autoantibodies. Identified candidate antigens were expressed in vitro to enable validation studies with radiobinding assays (RBA). The validation cohort included NT1 patients (n\u00a0=\u00a039), their first\u2010degree relatives (FDR) (n\u00a0=\u00a066), population controls (n\u00a0=\u00a0188), and disease controls representing multiple sclerosis (n\u00a0=\u00a0100) and FDR to type 1 diabetes patients (n\u00a0=\u00a041). Reactivity towards previously suggested NT1 autoantigen candidates including Tribbles homolog 2, Prostaglandin D2 receptor, Hypocretin receptor 2 and \u03b1\u2010MSH\/proopiomelanocortin was not replicated in the protein array screen. By comparing case to control signals, three novel candidate autoantigens were identified in the protein array screen; LOC401464, PARP3 and FAM63B. However, the RBA did not confirm elevated reactivity towards either of these proteins. In summary, three putative autoantigens in NT1 were identified by protein array screening. Autoantibodies against these candidates could not be verified with independent methods. Further studies are warranted to identify hypothetical autoantigens related to the pathogenesis of Pandemrix\u00ae\u2010induced NT1.<\/jats:p>","DOI":"10.1111\/sji.12864","type":"journal-article","created":{"date-parts":[[2020,2,14]],"date-time":"2020-02-14T07:43:02Z","timestamp":1581666182000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Screening for autoantibody targets in post\u2010vaccination narcolepsy using proteome arrays"],"prefix":"10.1111","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1473-5468","authenticated-orcid":false,"given":"Alexander","family":"Lind","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences Malm\u00f6 Lund University\/CRC Sk\u00e5ne University Hospital SUS Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Eriksson","sequence":"additional","affiliation":[{"name":"Department of Medicine (Solna) Center for Molecular Medicine Karolinska Institutet Stockholm Sweden"},{"name":"Department of Endocrinology, Metabolism and Diabetes Karolinska University Hospital Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar","family":"Akel","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Malm\u00f6 Lund University\/CRC Sk\u00e5ne University Hospital SUS Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita","family":"Ramelius","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Malm\u00f6 Lund University\/CRC Sk\u00e5ne University Hospital SUS Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Palm","sequence":"additional","affiliation":[{"name":"Section for Paediatric Neurology Department of Paediatrics Sk\u00e5ne University Hospital SUS Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c5ke","family":"Lernmark","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Malm\u00f6 Lund University\/CRC Sk\u00e5ne University Hospital SUS Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olle","family":"K\u00e4mpe","sequence":"additional","affiliation":[{"name":"Department of Medicine (Solna) Center for Molecular Medicine Karolinska Institutet Stockholm Sweden"},{"name":"Department of Endocrinology, Metabolism and Diabetes Karolinska University Hospital Stockholm Sweden"},{"name":"Science for Life Laboratory Department of Medical Sciences Uppsala University Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Elding Larsson","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Malm\u00f6 Lund University\/CRC Sk\u00e5ne University Hospital SUS Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nils","family":"Landegren","sequence":"additional","affiliation":[{"name":"Department of Medicine (Solna) Center for Molecular Medicine Karolinska Institutet Stockholm Sweden"},{"name":"K.G. 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