{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T03:10:08Z","timestamp":1781320208609,"version":"3.54.1"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T00:00:00Z","timestamp":1594684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Lipopolysaccharide (LPS)\u2010responsive beige\u2010like anchor (LRBA) protein was initially described as a monogenetic cause for common variable immune deficiency, a syndrome characterized by low levels of B cells, defects in memory B cell differentiation and hypogammaglobulinaemia. <jats:italic>LRBA<\/jats:italic> was identified as an LPS up\u2010regulated gene in B cells, macrophages and T cells. LRBA weighs 320\u00a0kDa and has 2863 amino acids. Its sequence contains multiple domains, suggesting that LRBA can act as a scaffolding protein. It contains two putative binding sites for cAMP\u2010dependent kinase (PKA) regulatory subunits, suggesting this protein can act as A\u2010kinase anchor protein (AKAP); however, physical interactions involving LRBA and PKA have not been demonstrated to date, and functional roles for such interactions are unexplored. In this work, we investigated physical interactions involving LRBA with regulatory subunits of PKA in human B cell lines and primary human B cells. PKA is a holoenzyme composed of two regulatory subunits, which can be RI\u03b1, RI\u03b2, RII\u03b1 or RII\u03b2, and two catalytic subunits, C\u03b1 or C\u03b2. We co\u2010immunoprecipitated LRBA using Ramos B cell lymphoma cells and observed that LRBA interacts with RII\u03b2. Interestingly, St\u2010Ht31, an inhibitory peptide that disrupts AKAP interactions with regulatory subunits, reduced the amount of interacting protein. Furthermore, in primary human B cells, LRBA was induced after CD40L and IL\u20104 stimulation, and under such activation, we found that LRBA interacts with RII\u03b1 and RII\u03b2, suggesting that LRBA acts as an AKAP and binds RII subunits. Interestingly, we also identified that LRBA interacts with activation\u2010induced cytidine deaminase in primary B cells, suggesting that it is involved in B cell function.<\/jats:p>","DOI":"10.1111\/sji.12922","type":"journal-article","created":{"date-parts":[[2020,6,27]],"date-time":"2020-06-27T10:25:26Z","timestamp":1593253526000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Lipopolysaccharide\u2010responsive beige\u2010like anchor acts as a cAMP\u2010dependent protein kinase anchoring protein in B cells"],"prefix":"10.1111","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5248-0454","authenticated-orcid":false,"given":"Nidia Carolina","family":"Moreno\u2010Corona","sequence":"first","affiliation":[{"name":"Biomedicina Molecular Centro de Investigacion y de Estudios Avanzados  Mexico City Mexico"},{"name":"Unidad de Investigacion en Inmunodeficiencias Instituto Nacional de Pediatria  Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0319-1661","authenticated-orcid":false,"given":"Orestes","family":"Lopez\u2010Ortega","sequence":"additional","affiliation":[{"name":"Biomedicina Molecular Centro de Investigacion y de Estudios Avanzados  Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1327-8082","authenticated-orcid":false,"given":"Jose Mizael","family":"Flores Hermenegildo","sequence":"additional","affiliation":[{"name":"Biomedicina Molecular Centro de Investigacion y de Estudios Avanzados  Mexico City Mexico"},{"name":"Unidad de Investigacion en Inmunodeficiencias Instituto Nacional de Pediatria  Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3290-8705","authenticated-orcid":false,"given":"Laura","family":"Berron\u2010Ruiz","sequence":"additional","affiliation":[{"name":"Unidad de Investigacion en Inmunodeficiencias Instituto Nacional de Pediatria  Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2824-3680","authenticated-orcid":false,"given":"Juan Carlos","family":"Rodriguez\u2010Alba","sequence":"additional","affiliation":[{"name":"Unidad de Citometria de Flujo Instituto de Ciencias de la Salud Universidad Veracruzana  Xalapa Veracruz Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4772-0713","authenticated-orcid":false,"given":"Leopoldo","family":"Santos\u2010Argumedo","sequence":"additional","affiliation":[{"name":"Biomedicina Molecular Centro de Investigacion y de Estudios Avanzados  Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5498-6739","authenticated-orcid":false,"given":"Gabriela","family":"Lopez\u2010Herrera","sequence":"additional","affiliation":[{"name":"Unidad de Investigacion en Inmunodeficiencias Instituto Nacional de Pediatria  Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2009.51"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa1663"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2017.52"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2017.50"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.09.025"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2012.04.015"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2018.08.020"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.7.4586"},{"key":"e_1_2_8_10_1","first-page":"6","article-title":"The role of BEACH proteins in Dictyostelium","volume":"1","author":"De Lozanne A","year":"2000","journal-title":"Traffic"},{"key":"e_1_2_8_11_1","first-page":"1","article-title":"Lipopolysaccharide\u2010responsive beige\u2010like anchor (LRBA), a novel regulator of human immune disorders","volume":"1","author":"Wang JW","year":"2014","journal-title":"Austin J Clin Immunol"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/tra.12069"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr000236l"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0898-6568(01)00214-5"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830260617"},{"key":"e_1_2_8_16_1","article-title":"Tasken K. 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