{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T03:51:28Z","timestamp":1772164288997,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,7,22]],"date-time":"2020-07-22T00:00:00Z","timestamp":1595376000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001732","name":"Danmarks Grundforskningsfond","doi-asserted-by":"publisher","award":["126"],"award-info":[{"award-number":["126"]}],"id":[{"id":"10.13039\/501100001732","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Major surgery is associated with substantial morbidity and mortality with early post\u2010operative adverse events (POAE) occurring in 30% of patients within the first 30\u00a0days. The underlying pathogenesis is multifactorial, including immune dysfunction and increased inflammatory response to surgery. We investigated preoperative immune function by the TruCulture\u00ae whole blood technique in a cohort of patients undergoing pancreaticoduodenectomy (PD), hypothesizing that patients developing inflammatory POAE defined as leucocytosis, fever or high (above median) area under the curve (AUC) C\u2010reactive protein (CRP) the first post\u2010operative week would display perturbed preoperative immune function. Sixty\u2010two adult patients were screened, 30 included and 11 excluded post\u2010inclusion due to other surgical procedures than PD and post\u2010operative complications directly attributed to surgery, leaving 19 patients for analysis of preoperative immune function. Patients developing leucocytosis (n\u00a0=\u00a05, 26%) had lower Toll\u2010like receptor (TLR)\u20103\u2013stimulated IL\u201012p40 and higher <jats:italic>Candida Albicans<\/jats:italic> (TLR1\/2\/4\/6, Dectin\u20101)\u2010stimulated TNF\u2010\u03b1, compared to patients without leucocytosis (all <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). Patients developing fever (n\u00a0=\u00a07, 37%) had lower TLR7\/8\u2010stimulated IFN\u2010\u03b3 and patients with high AUC CRP (n\u00a0=\u00a09, 47%) had lower TLR3\u2010stimulated IFN\u2010\u03b3 and IL\u20106 and lower TLR7\/8\u2010stimulated IL\u201010 (all <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05), compared to patients without fever or low CRP, respectively. In conclusion, patients with inflammatory POAE displayed lower preoperative stimulated IL\u201012p40, IFN\u2010\u03b3, IL\u20106 and IL\u201010 and higher TNF\u2010\u03b1 response, compared to patients without inflammatory POAE. This finding suggests that TruCulture is a feasible immunologic screening tool in surgical patients, with a potential for preoperative identification of patients at increased risk for inflammatory POAE, allowing for risk\u2010based intervention trials.<\/jats:p>","DOI":"10.1111\/sji.12930","type":"journal-article","created":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T19:46:58Z","timestamp":1594237618000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Preoperative TruCulture\u00ae whole blood cytokine response predicts post\u2010operative inflammation in pancreaticoduodenectomy patients\u2014A pilot cohort study"],"prefix":"10.1111","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7131-2461","authenticated-orcid":false,"given":"Eske K.","family":"Aasvang","sequence":"first","affiliation":[{"name":"Deptartment of Anesthesiology Centre for Cancer and Organ Diseases Rigshospitalet Copenhagen 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H.","family":"Storkholm","sequence":"additional","affiliation":[{"name":"Deptartment of Surgical Gastroenterology Centre for Cancer and Organ Diseases Rigshospitalet Copenhagen University Hospital Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul S.","family":"Krohn","sequence":"additional","affiliation":[{"name":"Deptartment of Surgical Gastroenterology Centre for Cancer and Organ Diseases Rigshospitalet Copenhagen University Hospital Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan K.","family":"Burgdorf","sequence":"additional","affiliation":[{"name":"Deptartment of Surgical Gastroenterology Centre for Cancer and Organ Diseases Rigshospitalet Copenhagen University Hospital Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jakob H.","family":"Von Stemann","sequence":"additional","affiliation":[{"name":"Deptartment of Clinical Immunology Centre for Diagnostic Investigation Copenhagen University Hospital Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens D.","family":"Lundgren","sequence":"additional","affiliation":[{"name":"Deptartment of Clinical Medicine Faculty of Health and Medical Sciences University of Copenhagen Copenhagen Denmark"},{"name":"Deptartment of Infectious Diseases Centre for Cancer and Organ Diseases Rigshospitalet Copenhagen University Hospital Copenhagen Denmark"},{"name":"PERSIMUNE Centre of Excellence for Personalized Medicine of Infections Complications in Immune Deficiency and CHIP Centre of Excellence for Health, Immunity and Infections Department of Infectious Diseases, Section 2100 Copenhagen University Hospital Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne D.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Deptartment of Clinical Medicine Faculty of Health and Medical Sciences University of Copenhagen 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Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2015.11.007"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0000000000001691"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-200204000-00015"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.5404"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.161.5.2655"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-6576.2010.02218.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-200301000-00006"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0000000000001248"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-030409-101317"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.03.002"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.08.011"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pan.2017.07.190"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.shk.0000228172.32587.7a"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/aas.13522"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/35099560"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni0602-499"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2018.03.002"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3009701"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0000000000000887"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2013.302"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1863"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00847"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2017.09.019"}],"container-title":["Scandinavian 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