{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:16:06Z","timestamp":1790612166026,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100002129","name":"Heart Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100002129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004885","name":"Ume\u00e5 Universitet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004885","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ankylosing spondylitis (AS) is an autoimmune disease affecting parts of the skeletal structure in particular. Previously increased levels of the inflammatory cell types Th17, Th22, Tc17 and Tc22 cells have been shown to be associated with AS. Here, we analysed the levels of inflammatory T cell subsets, related cytokines and clinical characteristics of AS patients vs controls from northern Sweden. Peripheral blood mononuclear cells (PBMCs) obtained from 50 AS patients and 50 matched controls were short term stimulated with PMA\/Ionomycin, stained and analysed by flow cytometry. Plasma levels of Interleukin (IL)\u201017, IL\u201022, IL\u201010 as well as clinically relevant markers were determined. Compared to male controls, male AS patients showed 1.5\u2010 to 2\u2010fold increases of Th17 (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.013), Th22 (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.003) and Tc22 (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.024) among CD45<jats:sup>+<\/jats:sup>CD3<jats:sup>+<\/jats:sup> lymphocytes. Plasma IL\u201022 levels correlated with the Tc17 proportion in male patients (R<jats:sub>s<\/jats:sub>\u00a0=\u00a00.499, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.003) and plasma IL\u201010 levels were inversely correlated with Tc17 among all patients (R<jats:sub>s<\/jats:sub>\u00a0=\u00a0\u22120.276, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.05). Male patients with syndesmophytes showed significantly higher Th17 proportions (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.038). In female AS patients, Tc22 was negatively correlated with C\u2010reactive protein (high sensitivity) (hsCRP) (R<jats:sub>s<\/jats:sub>\u00a0=\u00a0\u22120.573, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.016). We confirmed increased proportions of inflammatory T cells and correlations with relevant cytokines from male AS patients. The correlation between Th17 and syndesmophytes supports a role of Th17 in the pathogenic process.<\/jats:p>","DOI":"10.1111\/sji.13190","type":"journal-article","created":{"date-parts":[[2022,5,4]],"date-time":"2022-05-04T13:30:05Z","timestamp":1651671005000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Increased proportions of inflammatory T cells and their correlations with cytokines and clinical parameters in patients with ankylosing spondylitis from northern Sweden"],"prefix":"10.1111","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5025-6539","authenticated-orcid":false,"given":"Kristina","family":"Lejon","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology Ume\u00e5 University  Ume\u00e5 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Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7871-5303","authenticated-orcid":false,"given":"Helena","family":"Forsblad\u2010d\u2019Elia","sequence":"additional","affiliation":[{"name":"Division of Medicine, Department of Public Health and Clinical Medicine Section of Rheumatology, Ume\u00e5 University  Ume\u00e5 Sweden"},{"name":"Department of Rheumatology and Inflammation Research Institute of Medicine, Sahlgrenska Academy, University of Gothenburg  Gothenburg 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