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We used a mouse model of BCG skin infection to study the effect of BCG dose on the relocation of skin Dendritic cells (DCs) to draining lymph node (DLN). <jats:italic>Mycobacterium<\/jats:italic> antigen 85B\u2010specific CD4<jats:sup>+<\/jats:sup> P25 T cell\u2010receptor transgenic (P25 TCRTg) cells were used to probe priming to BCG in DLN. DC migration and T cell priming were studied across BCG inocula that varied up to 100\u2010fold (10<jats:sup>4<\/jats:sup> to 10<jats:sup>6<\/jats:sup> Colony\u2010forming units\u2014CFUs). In line with earlier results in guinea pigs, DTH reaction in our model correlated with BCG dose. Importantly, priming of P25 TCRTg cells in DLN also escalated in a dose\u2010dependent manner, peaking at day 6 after infection. Similar dose\u2010escalation effects were seen for DC migration from infected skin and the accompanying transport of BCG to the DLN. BCG\u2010triggered upregulation of co\u2010stimulatory molecules on migratory DCs was restricted to the first 24\u2009hour after infection and was independent of BCG dose over a 10\u2010fold range (10<jats:sup>5<\/jats:sup> to 10<jats:sup>6<\/jats:sup> CFUs). The dose seemed to be a determinant of the number of total skin DCs that move to the DLN. In summary, our results support the use of higher BCG doses to detect robust DC migration and T cell priming.<\/jats:p>","DOI":"10.1111\/sji.13342","type":"journal-article","created":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T12:12:45Z","timestamp":1700655165000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["BCG infection dose guides dendritic cell migration and T cell priming in the draining lymph node"],"prefix":"10.1111","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8094-6266","authenticated-orcid":false,"given":"Veronika","family":"Krmesk\u00e1","sequence":"first","affiliation":[{"name":"Department of Microbiology Tumor and Cell Biology (MTC) Karolinska Institutet  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Microbiology Tumor and Cell Biology (MTC) Karolinska Institutet  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne","family":"Nyl\u00e9n","sequence":"additional","affiliation":[{"name":"Department of Microbiology Tumor and Cell Biology (MTC) Karolinska Institutet  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pryscilla Fanini","family":"Wowk","sequence":"additional","affiliation":[{"name":"Department of Microbiology Tumor and Cell Biology (MTC) Karolinska Institutet  Stockholm Sweden"},{"name":"Instituto Carlos Chagas Funda\u00e7\u00e3o Oswaldo Cruz (ICC\/Fiocruz\u2010PR)  Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6001-7240","authenticated-orcid":false,"given":"Antonio Gigliotti","family":"Rothfuchs","sequence":"additional","affiliation":[{"name":"Department of Microbiology Tumor and Cell Biology (MTC) Karolinska Institutet  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,11,21]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_10_2_1","DOI":"10.1016\/B978-0-12-417028-5.00002-8"},{"doi-asserted-by":"publisher","key":"e_1_2_10_3_1","DOI":"10.1371\/journal.ppat.1005206"},{"doi-asserted-by":"publisher","key":"e_1_2_10_4_1","DOI":"10.1016\/S1473-3099(21)00403-5"},{"doi-asserted-by":"publisher","key":"e_1_2_10_5_1","DOI":"10.1016\/j.vaccine.2019.11.060"},{"doi-asserted-by":"publisher","key":"e_1_2_10_6_1","DOI":"10.1038\/nrurol.2014.15"},{"doi-asserted-by":"publisher","key":"e_1_2_10_7_1","DOI":"10.1016\/S0022-5347(17)58737-6"},{"doi-asserted-by":"publisher","key":"e_1_2_10_8_1","DOI":"10.1146\/annurev.immunol.021908.132703"},{"doi-asserted-by":"publisher","key":"e_1_2_10_9_1","DOI":"10.1016\/j.vaccine.2015.08.033"},{"doi-asserted-by":"publisher","key":"e_1_2_10_10_1","DOI":"10.1016\/S0140-6736(10)60393-5"},{"doi-asserted-by":"publisher","key":"e_1_2_10_11_1","DOI":"10.1001\/jama.1994.03510330076038"},{"doi-asserted-by":"publisher","key":"e_1_2_10_12_1","DOI":"10.1016\/S0140-6736(06)68507-3"},{"key":"e_1_2_10_13_1","first-page":"31","article-title":"Experimental studies of vaccination, allergy, and immunity in tuberculosis. 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