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However, a subset of patients does not benefit from CAR\u2010T therapy. Our study aims to identify predictive indicators and establish a model to evaluate the feasibility of CAR\u2010T therapy. Fifty\u2010five R\/R B\u2010ALL patients and 22 healthy donors were enrolled. Peripheral blood lymphocyte subsets were analysed using flow cytometry. Sensitivity, specificity, accuracy, positive and negative predictive values and receiver operating characteristic (ROC) areas under the curve (AUC) were determined to evaluate the predictive values of the indicators. We identified B lymphocyte, regulatory T cell (Treg) and peripheral blood minimal residual leukaemia cells (B\u2010MRD) as indicators for predicting the success of CAR\u2010T cell preparation with AUC 0.936, 0.857 and 0.914. Furthermore, a model based on CD3<jats:sup>+<\/jats:sup> T count, CD4<jats:sup>+<\/jats:sup> T\/CD8<jats:sup>+<\/jats:sup> T ratio, Treg and extramedullary diseases (EMD) was used to predict the response to CAR\u2010T therapy with AUC of 0.938. Notably, a model based on CD4<jats:sup>+<\/jats:sup> T\/CD8<jats:sup>+<\/jats:sup> T ratio, B, Treg and EMD were used in predicting the success of CAR\u2010T therapy with AUC 0.966 [0.908\u20131.000], with specificity (92.59%) and sensitivity (91.67%). In the validated group, the predictive model predicted the success of CAR\u2010T therapy with specificity (90.91%) and sensitivity (100%). We have identified several predictive indicators for CAR\u2010T cell therapy success and a model has demonstrated robust predictive capacity for the success of CAR\u2010T therapy. These results show great potential for guiding informed clinical decisions in the field of CAR\u2010T cell therapy.<\/jats:p>","DOI":"10.1111\/sji.13352","type":"journal-article","created":{"date-parts":[[2024,1,17]],"date-time":"2024-01-17T05:53:25Z","timestamp":1705470805000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Predictive model for <scp>CAR\u2010T<\/scp> cell therapy success in patients with relapsed\/refractory B\u2010cell acute lymphoblastic leukaemia"],"prefix":"10.1111","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0795-8396","authenticated-orcid":false,"given":"Lianfang","family":"Pu","sequence":"first","affiliation":[{"name":"Department of Hematology\/Hematological Lab The Second Affiliated Hospital of Anhui Medical University  Hefei Anhui China"},{"name":"Center of Hematology Research Anhui Medical University  Hefei Anhui 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