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Such modifications may generate neo\u2010epitopes, break immunological tolerance, and induce antibody response. In this study, we have first analysed the structural modifications of whole histone in the presence of deoxyribose followed by oxidation with hydroxyl radicals. Changes in the secondary and tertiary structure of the whole histone were determined by spectroscopic techniques and biochemical assays. Fluorescence spectroscopy and UPLC\u2013MS showed the generation of AGEs such as carboxymethyl lysine and pentosidine, while DLS and TEM indicated the presence of amorphous AGE\u2010aggregates. Moreover, rabbits immunized with these histone\u2010AGEs exhibited enhanced immunogenicity and ELISA and western immunoblot of IgG antibodies from SLE patients' sera showed a significantly higher specificity towards modified histone\u2010AGEs than the native histone.<\/jats:p>","DOI":"10.1111\/sji.13389","type":"journal-article","created":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T04:09:39Z","timestamp":1717128579000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Glycoxidation of mammalian whole histone generates highly immunogenic aggregates: Sera of SLE patients contain autoantibodies against aggregates"],"prefix":"10.1111","volume":"100","author":[{"given":"Shireen Naaz","family":"Islam","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Jawaharlal Nehru Medical College, Faculty of Medicine Aligarh Muslim University  Aligarh UP India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zarina","family":"Arif","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Jawaharlal Nehru Medical College, Faculty of Medicine Aligarh Muslim University  Aligarh UP India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asim","family":"Badar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Jawaharlal Nehru Medical College, Faculty of Medicine Aligarh Muslim University  Aligarh UP India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Moinuddin","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Jawaharlal Nehru Medical College, Faculty of Medicine Aligarh Muslim University  Aligarh UP India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md. 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