{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:41:37Z","timestamp":1753879297331,"version":"3.41.2"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T00:00:00Z","timestamp":1742860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82473530","82173341"],"award-info":[{"award-number":["82473530","82173341"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Systemic lupus erythematosus (SLE) is a multi\u2010system autoimmune disease characterised by aberrant activation and differentiation of autoreactive T and B cells, as well as the overproduction of autoantibodies. CD6, a cell\u2010surface glycoprotein, regulates lymphocyte activation, differentiation and survival, and is implicated in the pathogenesis of various autoimmune disorders. In SLE, the CD6\/activated leukocyte cell adhesion molecule (ALCAM) pathway promotes renal T\u2010cell immune responses. However, the distribution, expression, and function of CD6 in lupus B cells remain poorly understood. In this work, we employed flow cytometry and multi\u2010colour immunohistochemical staining to analyse the expression and distribution of CD6 on peripheral B cells. Correlation analysis was performed to assess the associations of CD6 and clinical indicators of disease severity. We found that SLE patients exhibited significantly reduced CD6 expression on peripheral CD19<jats:sup>+<\/jats:sup> B, CD19<jats:sup>+<\/jats:sup>CD27<jats:sup>\u2212<\/jats:sup> B, CD19<jats:sup>+<\/jats:sup>CD27<jats:sup>+<\/jats:sup> B, na\u00efve B, CD19<jats:sup>+<\/jats:sup>CD27<jats:sup>\u2212<\/jats:sup>IgD<jats:sup>\u2212<\/jats:sup> double\u2010negative B (DNB) and CD19<jats:sup>+<\/jats:sup>CD27<jats:sup>+<\/jats:sup>IgD<jats:sup>+<\/jats:sup> B cells. Moreover, CD6 expression was negatively correlated with serum levels of alanine transaminase (ALT), lactate dehydrogenase (LDH) and the degree of white blood cell (WBC) depletion. Notably, SLE patients positive for antinuclear antibody (ANA) or anti\u2010SSA antibody displayed lower CD6 expression on circulating B cells. Additionally, CD6 expression in B cells was predominantly localised in the extrafollicular (EF) region of human tonsils, suggesting a potential regulatory role of CD6 in EF B\u2010cell responses. In conclusion, dysregulated CD6 expression on peripheral B cells might be related to liver\/kidney injury and ANA\/anti\u2010SSA antibody production in SLE patients.<\/jats:p>","DOI":"10.1111\/sji.70018","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T21:28:29Z","timestamp":1743197309000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Aberrant Lower <scp>CD6<\/scp> Expression on Peripheral B Cells Associated With Liver\/Kidney Injury and Autoantibody Production of Systemic Lupus Erythematosus Patients"],"prefix":"10.1111","volume":"101","author":[{"given":"Yongjian","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Dermatology Hunan Provincial People's Hospital  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Mei","sequence":"additional","affiliation":[{"name":"Department of Dermatology Second Xiangya Hospital, Central South University, Hunan Key Laboratory of Medical Epigenomics  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Dermatology Second Xiangya Hospital, Central South University, Hunan Key Laboratory of Medical Epigenomics  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fen","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Dermatology Second Xiangya Hospital, Central South University, Hunan Key Laboratory of Medical Epigenomics  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoran","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Second Xiangya Hospital, Central South University, Hunan Key Laboratory of Medical Epigenomics  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miao","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Second Xiangya Hospital, Central South University, Hunan Key Laboratory of Medical Epigenomics  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaxiong","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Dermatology Second Xiangya Hospital, Central South University, Hunan Key Laboratory of Medical Epigenomics  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianwen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology Second Xiangya Hospital, Central South University, Hunan Key Laboratory of Medical Epigenomics  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gangcai","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Second Xiangya Hospital, Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3213-6717","authenticated-orcid":false,"given":"Ping","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Radiology Xiangya Hospital, Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Second Xiangya Hospital, Central South University, Hunan Key Laboratory of Medical Epigenomics  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,25]]},"reference":[{"volume-title":"StatPearls","year":"2024","author":"Justiz Vaillant A. A.","key":"e_1_2_10_2_1"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(24)00398-2"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2017.12.004"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1704008114"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.2.1152"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1615253114"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.749804"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2024.103228"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.41288"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2010.07.005"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI147334"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.966184"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15986-3"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-024-06883-x"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-021-00914-4"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)00405-7"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.4.2262"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200424856"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-09-3881"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201040528"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20151785"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1416276112"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.202217341"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.08.015"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00769"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/0961203320923398"},{"issue":"6","key":"e_1_2_10_28_1","article-title":"Liver Abnormalities in Systemic Lupus Erythematosus: A Prospective Observational Study","volume":"13","author":"Imran S.","year":"2021","journal-title":"Cureus"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2024.103188"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00594"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2023.109710"}],"container-title":["Scandinavian Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/sji.70018","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T13:13:21Z","timestamp":1748351601000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/sji.70018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,25]]},"references-count":30,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1111\/sji.70018"],"URL":"https:\/\/doi.org\/10.1111\/sji.70018","archive":["Portico"],"relation":{},"ISSN":["0300-9475","1365-3083"],"issn-type":[{"type":"print","value":"0300-9475"},{"type":"electronic","value":"1365-3083"}],"subject":[],"published":{"date-parts":[[2025,3,25]]},"assertion":[{"value":"2025-03-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70018"}}