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Platelet\u2010activating factor (PAF) is central in thromboinflammatory processes. However, its role in the interaction of platelets and neutrophils requires further insights. Therefore, we elucidated PAF\u2010related neutrophil activation, including platelet\u2013neutrophil complex (PNC) formation\u00a0and investigated potential strategies to modulate PAF\u2010related inflammation. For the translation of the PAF\u2010mediated inflammation, we applied an animal\u2010free human ex\u00a0vivo whole blood model. The neutrophil phenotype, its function, and PNC formation were studied by flow cytometry and platelet\u2010related activity was assessed by light microscopy and aggregometry. PAF induced a rapid and dose\u2010dependent change in neutrophil phenotype, as evidenced by CD10, CD11b, and CD66b upregulation and CD62L downregulation. Moreover, PAF increased the generation of reactive oxygen species (ROS), phagocytic activity and PNC formation. Interestingly, PNCs displayed significantly enhanced ROS formation and phagocytosis compared to neutrophils without attached platelets, whereas these differences were not observed regarding phenotype changes. Furthermore, the findings were confirmed in a clinically relevant ex\u00a0vivo whole blood model of lipopolysaccharide\u2010 or PAF\u2010driven inflammation. In summary, the present study elucidates PAF\u2010driven effects on neutrophils and their interaction with platelets. The findings might help in developing therapeutic approaches to modulate PAF\u2010related thromboinflammation, for example, during sepsis.<\/jats:p>","DOI":"10.1111\/sji.70044","type":"journal-article","created":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T04:00:57Z","timestamp":1753761657000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Platelet\u2010Activating Factor Promotes Neutrophil Activation and Platelet\u2013Neutrophil Complex Formation"],"prefix":"10.1111","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5795-812X","authenticated-orcid":false,"given":"Lisa","family":"Wohlgemuth","sequence":"first","affiliation":[{"name":"Institute of Clinical and Experimental Trauma Immunology University Hospital of Ulm  Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiane Leonie","family":"Knapp","sequence":"additional","affiliation":[{"name":"Institute of 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