{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:16:31Z","timestamp":1758672991364,"version":"3.44.0"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:00:00Z","timestamp":1756080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["FileNo","5\/7\/1654\/CH\/Adhoc\/2019\u2010RBMCH(I\u20101107)"],"award-info":[{"award-number":["FileNo","5\/7\/1654\/CH\/Adhoc\/2019\u2010RBMCH(I\u20101107)"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Juvenile idiopathic arthritis (JIA) encompasses distinct inflammatory subtypes such as polyarticular (pJIA), oligoarticular (oJIA), systemic onset (sJIA) and enthesitis\u2010related arthritis (ERA). The molecular mechanisms underlying these subtypes remain unclear. This study aimed to investigate the differential protein expression in synovial fluid (SF) across these subtypes of JIA using high\u2010throughput proteomics to identify potential diagnostic biomarkers. SF samples were collected from 48 children (45 JIA and 3 non\u2010inflammatory controls). Samples underwent protein extraction, iTRAQ labelling and LC\u2013MS\/MS analysis. A total of 282 proteins were identified. Differentially expressed proteins were analysed using fold change (FC) relative to control samples within each iTRAQ set. Principal component analysis (PCA), gene ontology (GO) and KEGG pathway enrichment analyses were performed to assess the biological significance of the identified proteins. Proteins showing &gt;\u20095FC were prioritised for clustering and biomarker identification. Distinct proteomic signatures were observed across the studied JIA subtypes. PCA revealed the heterogeneity of protein expression in different subtypes of JIA. S\u2010100, Septin\u20107, MMP\u201016 proteins were commonly upregulated in the studied subtypes of JIA except oJIA. Haptoglobin was upregulated in all except sJIA. The specific proteins expressed in various subtypes include pyruvate dehydrogenase phosphate in pJIA, haptoglobin\u2010related and fibrinogen proteins in ERA, apolipoprotein and Nck\u2010associated protein in sJIA and leucine\u2010rich alpha\u20102\u2010glycoprotein in oJIA. GO analysis revealed enrichment in immune\u2010related biological processes, including complement activation, humoral immune response and antigen binding. The differential SF proteomic signatures observed in the studied JIA subtypes indicate different pathogenic mechanisms in JIA.<\/jats:p>","DOI":"10.1111\/sji.70051","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T03:53:58Z","timestamp":1756180438000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synovial Fluid Proteomic Signatures in Different Subtypes of Juvenile Idiopathic Arthritis"],"prefix":"10.1111","volume":"102","author":[{"given":"Narendra Kumar","family":"Bagri","sequence":"first","affiliation":[{"name":"Division of Pediatric Rheumatology, Department of Pediatrics All India Institute of Medical Sciences (AIIMS)  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saumya","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Division of Pediatric Rheumatology, Department of Pediatrics All India Institute of Medical Sciences (AIIMS)  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yogendra","family":"Singh","sequence":"additional","affiliation":[{"name":"Division of Pediatric Rheumatology, Department of Pediatrics All India Institute of Medical Sciences (AIIMS)  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Revathy","family":"Neelamegan","sequence":"additional","affiliation":[{"name":"Division of Pediatric Rheumatology, Department of Pediatrics All India Institute of Medical Sciences (AIIMS)  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashish","family":"Upadhyay","sequence":"additional","affiliation":[{"name":"Central Research Unit (CRU) AIIMS  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Venkatesan S.","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics AIIMS  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4757-8729","authenticated-orcid":false,"given":"Subhradip","family":"Karmakar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry AIIMS  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Chew","sequence":"additional","affiliation":[{"name":"Department of Paediatric Rheumatology Bristol Royal Hospital for Children  Bristol UK"},{"name":"School of Cellular and Molecular Medicine University of Bristol  Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. V.","family":"Ramanan","sequence":"additional","affiliation":[{"name":"Department of Paediatric Rheumatology Bristol Royal Hospital for Children  Bristol UK"},{"name":"School of Cellular and Molecular Medicine University of Bristol  Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0334-5197","authenticated-orcid":false,"given":"Thirumurthy","family":"Velpandian","sequence":"additional","affiliation":[{"name":"Division of Ocular Pharmacology and Pharmacy, Dr RP Centre for Ophthalmic Sciences AIIMS  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,25]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2018-213030"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2614"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1134747"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1055\/a-1012-8571"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.27447"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200600422"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3356"},{"key":"e_1_2_11_9_1","first-page":"390","article-title":"International League of Associations for Rheumatology Classification of Juvenile Idiopathic Arthritis: Second Revision, Edmonton, 2001","volume":"31","author":"Petty R. 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