{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:17:02Z","timestamp":1758673022628,"version":"3.44.0"},"reference-count":52,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["1004627"],"award-info":[{"award-number":["1004627"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The initial aim of this study on Balb\/C mice was to investigate the putative effects on feeding and appetite of isopentenyl pyrophosphate (IPP) and E\u20104\u2010hydroxy\u20103\u2010methyl\u2010but\u20102\u2010enyl pyrophosphate (HMBPP), also known as phosphoantigens (pAgs). HMBPP was recently shown to increase blood meal appetite in malaria mosquitoes. Both IPP and HMBPP are metabolites produced by the normal gut microbiota and apicomplexan parasites such as <jats:italic>Plasmodium<\/jats:italic>. To explore potential effects on appetite, male and female mice were treated by gavage with these metabolites, and body mass and gene expression were monitored in brain, stomach and small intestine at 3\u2009h and 7\u2009weeks. Body mass gain did not clearly differ between pAg\u2010treated and water control mice. However, beginning between 4 and 7\u2009weeks, the salivary glands of IPP\u2010treated males began to swell. With the autoimmune Sj\u00f6gren disease (SjD) in mind, we subsequently investigated the salivary glands after 1, 4 and 7\u2009weeks of IPP treatment. Fast gene set enrichment analysis (FGSEA) of marginal zone B\u2010cell (MZB) transcripts from salivary glands, together with B\u2010cell infiltration in both sexes at 4\u2009weeks, suggested similarities to SjD pathology. Using ELISA, we measured serum autoantibodies against Ro52, Ro60 and La. Multivariate analysis at 7\u2009weeks showed treatment\u2010associated trends: levels of anti\u2010Ro52 and anti\u2010La tended to increase in IPP\u2010treated males, but not in females. Notably, IL\u20106 serum levels displayed a sex\u2010dependent pattern, and PCA analyses of transcriptomic data from brain, stomach and small intestine\u2014though with some exceptions\u2014also indicated differential responses to pAgs between males and females.<\/jats:p>","DOI":"10.1111\/sji.70052","type":"journal-article","created":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T05:00:24Z","timestamp":1756875624000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sex Dependent and Sj\u00f6gren Disease Like Immune Responses Against Phosphoantigens in Balb\/C Mice"],"prefix":"10.1111","volume":"102","author":[{"given":"Paulina","family":"Czwakiel","sequence":"first","affiliation":[{"name":"Department of Molecular Biosciences, the Wenner\u2010Gren Institute Stockholm University  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bj\u00f6rn","family":"Brindefalk","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, the Wenner\u2010Gren Institute Stockholm University  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atiyeh","family":"Eghbali","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, the Wenner\u2010Gren Institute Stockholm University  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heinrich","family":"Dircksen","sequence":"additional","affiliation":[{"name":"Department of Zoology Stockholm University  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0968-7454","authenticated-orcid":false,"given":"Kahkashan","family":"Kamal","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, the Wenner\u2010Gren Institute Stockholm University  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahra","family":"Payandeh","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, the Wenner\u2010Gren Institute Stockholm University  Stockholm Sweden"},{"name":"Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5215-8684","authenticated-orcid":false,"given":"Deniz","family":"Ozata","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, the Wenner\u2010Gren Institute Stockholm University  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marita","family":"Troye\u2010Blomberg","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, the Wenner\u2010Gren Institute Stockholm University  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4382-7238","authenticated-orcid":false,"given":"Ingrid","family":"Faye","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, the Wenner\u2010Gren Institute Stockholm University  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9061433"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2006.00479.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109359"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.894315"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.202451068"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/322836a0"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.891687"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2950517"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.6b00537"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0073868"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aah4563"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1124234"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.051599-0"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.13740"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jobcr.2020.12.001"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.980400"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2020.102686"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22041919"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4197"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v067.i01"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00318163"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.079472.122"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2017.05.001"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/sji.12920"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241512209"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2022.109390"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2019.11.024"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep34019"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes15010028"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.01273"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110069"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-019-03642-4"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.115938"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-med-042320-014032"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-022-00700-9"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10030564"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00813"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23020992"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.23036"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2020.115327"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2012.01.004"},{"issue":"1","key":"e_1_2_9_45_1","first-page":"29","article-title":"Lifetime Reproductive Efficiency of BALB\/c Mouse Pairs After an Environmental Modification at 3 Mating Ages","volume":"54","author":"Moreira V. B.","year":"2015","journal-title":"Journal of the American Association for Laboratory Animal Science"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0012496623700539"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.90"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.712688"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07789-z"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kez052"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1136\/rmdopen-2023-003559"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12902"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/8959687"}],"container-title":["Scandinavian Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/sji.70052","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T12:54:20Z","timestamp":1758632060000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/sji.70052"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":52,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1111\/sji.70052"],"URL":"https:\/\/doi.org\/10.1111\/sji.70052","archive":["Portico"],"relation":{},"ISSN":["0300-9475","1365-3083"],"issn-type":[{"type":"print","value":"0300-9475"},{"type":"electronic","value":"1365-3083"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"2024-10-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70052"}}