{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T03:00:54Z","timestamp":1785380454379,"version":"3.55.0"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T00:00:00Z","timestamp":1767312000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["05991"],"award-info":[{"award-number":["05991"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2023\u201002312"],"award-info":[{"award-number":["2023\u201002312"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["20854"],"award-info":[{"award-number":["20854"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Leukotriene B\n                    <jats:sub>4<\/jats:sub>\n                    (LTB\n                    <jats:sub>4<\/jats:sub>\n                    ), a potent chemotactic and immune\u2010modulating eicosanoid, signals via two receptors (BLT\n                    <jats:sub>1<\/jats:sub>\n                    and BLT\n                    <jats:sub>2<\/jats:sub>\n                    ), leading to rapid but transient migratory, adhesive and secretory responses in phagocytes. Previously, we reported that BLT\n                    <jats:sub>1<\/jats:sub>\n                    is the predominating BLT in human umbilical vein endothelial cells (HUVEC). However, little is known about how ligation of these receptors affects adhesive and secretory endothelial responses over time. Here, we demonstrate that in HUVEC, LTB\n                    <jats:sub>4<\/jats:sub>\n                    dose\u2010dependently and stereospecifically causes a biphasic tethering of neutrophils, where the second phase is robust, similar to that induced by lipopolysaccharide, and persists for 3\u20138\u2009h. LTB\n                    <jats:sub>4<\/jats:sub>\n                    also causes up\u2010regulation of E\u2010selectin, ICAM\u20101 and VCAM\u20101 and release of MCP\u20101 and nitric oxide (but not of IL\u20108 or HMGB1). These responses appeared to be mediated via BLT\n                    <jats:sub>1<\/jats:sub>\n                    and BLT\n                    <jats:sub>2<\/jats:sub>\n                    as judged by BLT\n                    <jats:sub>1<\/jats:sub>\n                    shRNA gene silencing and\/or treatment with BLT\n                    <jats:sub>1<\/jats:sub>\n                    and BLT\n                    <jats:sub>2<\/jats:sub>\n                    specific antagonists prior to LTB\n                    <jats:sub>4<\/jats:sub>\n                    activation of HUVEC. Moreover, LTB\n                    <jats:sub>4<\/jats:sub>\n                    responses used primarily the MAP kinase\/Erk pathway. Our findings suggest a new role for LTB\n                    <jats:sub>4<\/jats:sub>\n                    not only in early but also in late vascular inflammatory responses.\n                  <\/jats:p>","DOI":"10.1111\/sji.70083","type":"journal-article","created":{"date-parts":[[2026,1,3]],"date-time":"2026-01-03T04:09:37Z","timestamp":1767413377000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>LTB4<\/scp>\n                    Activates the\n                    <scp>MAP<\/scp>\n                    Kinase Pathway in Endothelial Cells to Cause Long\u2010Lasting Neutrophil Tethering,\n                    <scp>MCP<\/scp>\n                    \u20101 and\n                    <scp>NO<\/scp>\n                    Releases"],"prefix":"10.1111","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4999-2058","authenticated-orcid":false,"given":"Anne\u2010Sofie","family":"Johansson","sequence":"first","affiliation":[{"name":"Center for Hematology and Regenerative Medicine  Stockholm Sweden"},{"name":"Department of Medicine Huddinge Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesper Z.","family":"Haeggstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Biophysics, Division of Chemistry II Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Palmblad","sequence":"additional","affiliation":[{"name":"Department of Medicine Huddinge Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,2]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6301011"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.13196"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R400027200"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-02-003665"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.125.4.1789"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1980.tb06696.x"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12859"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1996.tb08302.x"},{"issue":"2","key":"e_1_2_11_10_1","first-page":"132","article-title":"Stimulus\u2010Specific Neutrophil Aggregation: Evaluation of Possible Mechanisms for the Stimulus\u2010Response Apparatus","volume":"106","author":"Ringertz B.","year":"1985","journal-title":"Journal of Laboratory and Clinical Medicine"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.152.1.262"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1992.tb07946.x"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0602208103"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2010.07.016"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3006674"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(90)90887-S"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2796.2003.01204.x"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/niox.2000.0319"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1990.tb14151.x"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0b013e3181faceb3"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.7.2191"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plefa.2010.11.003"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.149.6.2163"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0300-9475.2004.01518.x"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1086\/322985"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0505845102"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvy130"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.545616"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000140063.06341.09"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-90636-2"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.163.11.6148"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2007.02743.x"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.5.3439"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI43302"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvn277"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M610540200"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1071550"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2820625"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.6.3887"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1988.tb13971.x"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)66589-5"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0214869"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1001031"}],"container-title":["Scandinavian Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/sji.70083","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/sji.70083","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/sji.70083","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T12:54:48Z","timestamp":1769000088000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/sji.70083"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1111\/sji.70083"],"URL":"https:\/\/doi.org\/10.1111\/sji.70083","archive":["Portico"],"relation":{},"ISSN":["0300-9475","1365-3083"],"issn-type":[{"value":"0300-9475","type":"print"},{"value":"1365-3083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-04-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70083"}}