{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:44:48Z","timestamp":1788345888378,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T00:00:00Z","timestamp":1769212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T00:00:00Z","timestamp":1769212800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000014","name":"Canadian Blood Services","doi-asserted-by":"publisher","award":["IG2018\u2010JA"],"award-info":[{"award-number":["IG2018\u2010JA"]}],"id":[{"id":"10.13039\/501100000014","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Adverse reactions from donor\u2010recipient sex\u2010mismatched transfusions have been reported in the field of transfusion medicine. Immature CD71\n                    <jats:sup>+<\/jats:sup>\n                    red blood cells (CD71\n                    <jats:sup>+<\/jats:sup>\n                    RBCs) may influence these reactions through an immunomodulatory mechanism potentially linked to enriched intracellular reactive oxygen species (ROS). This study was conducted to investigate the effect of CD71\n                    <jats:sup>+<\/jats:sup>\n                    RBCs on erythrophagocytosis, a common in\u00a0vitro test for hemolysis risk in incompatible blood transfusions. Enriched CD71\n                    <jats:sup>+<\/jats:sup>\n                    RBCs and CD71\n                    <jats:sup>\u2212<\/jats:sup>\n                    RBCs were exposed to allogenic peripheral blood mononuclear cells (PBMCs). CD71\n                    <jats:sup>+<\/jats:sup>\n                    RBCs were also incubated with anti\u2010D sensitised CD71\n                    <jats:sup>\u2212<\/jats:sup>\n                    RBCs and PBMCs, in varying ratios, to assess any dose\u2010dependent behaviour. An ROS scavenger, apocynin, was applied to understand how CD71\n                    <jats:sup>+<\/jats:sup>\n                    RBCs impact erythrophagocytosis. The phagocytosis index (RBC PI or CD71\n                    <jats:sup>+<\/jats:sup>\n                    RBC PI) was measured using image flow cytometry. Supernatant haemoglobin was examined spectrophotometrically. The enriched CD71\n                    <jats:sup>+<\/jats:sup>\n                    RBC group showed a significantly higher RBC PI and a reduction in the monocyte number compared to the CD71\n                    <jats:sup>\u2212<\/jats:sup>\n                    RBC group. Compared to the non\u2010treated group, the reduction of monocyte number and the increase of the phagocytosis index of CD71\n                    <jats:sup>+<\/jats:sup>\n                    RBCs was partially reversed in the apocynin\u2010treated group. There were positive correlations between the dose of CD71\n                    <jats:sup>+<\/jats:sup>\n                    RBC and CD71\n                    <jats:sup>+<\/jats:sup>\n                    RBC PI and supernatant haemoglobin. Donated CD71\n                    <jats:sup>+<\/jats:sup>\n                    RBCs enhance in\u00a0vitro erythrophagocytosis of CD71\n                    <jats:sup>+<\/jats:sup>\n                    RBCs in a dose\u2010dependent manner and reduce monocyte number. This work contributes to our understanding of immature CD71\n                    <jats:sup>+<\/jats:sup>\n                    RBCs' role in post\u2010transfusion immunobiology.\n                  <\/jats:p>","DOI":"10.1111\/sji.70087","type":"journal-article","created":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T02:04:20Z","timestamp":1769306660000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>CD71<\/scp>\n                    <sup>+<\/sup>\n                    Red Blood Cells Mediate Increased Erythrophagocytosis and Reduce Monocyte Number in a Monocyte Suspension Assay"],"prefix":"10.1111","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0230-8677","authenticated-orcid":false,"given":"Wenhui","family":"Li","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Alberta  Edmonton Alberta Canada"},{"name":"Department of Pathology Brigham and Women's Hospital, Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1445-827X","authenticated-orcid":false,"given":"Jason P.","family":"Acker","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Alberta  Edmonton Alberta Canada"},{"name":"Innovation and Portfolio Management, Canadian Blood Services  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,24]]},"reference":[{"issue":"2","key":"e_1_2_12_2_1","first-page":"S10","article-title":"Transfusion\u2010Associated Adverse Events and Implementation of Blood Safety Measures \u2013 Findings From the 2017 National Blood Collection and Utilization Survey","volume":"60","author":"Savinkina A. 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