{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T18:04:34Z","timestamp":1777658674027,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T00:00:00Z","timestamp":1769212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T00:00:00Z","timestamp":1769212800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Treatment of periodontitis may lower the inflammation status as measured in sera from patients. To investigate this in a biological assay, we co\u2010cultured periodontal ligament fibroblasts (PDLFs) from a healthy donor and peripheral blood mononuclear cells (PBMCs) as a source for osteoclasts. PDLFs were primed for 3\u2009days with serum from periodontitis patients before and from 1\u2009year after successful treatment of periodontitis (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200913). After priming, PBMCs were added. No significant differences in the number of osteoclasts that formed after 3\u2009weeks were found between baseline and after\u2010treatment serum priming. To find an explanation, we analysed the serum composition on the presence of inflammatory, pro\u2010osteoclastogenic cytokines. We found no differences between before and after treatment for cytokines MCP\u20101, TNF\u2010\u03b1, and IL\u20106. Interestingly, these levels of cytokines at baseline and after treatment strongly correlated per individual (correlation\n                    <jats:italic>p<\/jats:italic>\n                    \u2010value between\n                    <jats:italic>p\u2009=\u20090.03<\/jats:italic>\n                    and\n                    <jats:italic>p\u2009&lt;\u20090.0001<\/jats:italic>\n                    , correlation\n                    <jats:italic>r<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    between 0.37 and 0.89). Strikingly, between each other, MCP\u20101, TNF\u2010\u03b1, and IL\u20106 did not correlate. These results show that over the 1\u2010year time frame a cytokine equilibrium, the relatively similar levels of cytokines over a longer period, exists that is unique per individual. This suggests that the immune system is a constant for these cytokines, adapted to the needs of the individual. Larger cohorts, the inclusion of healthy individuals and longer time intervals need to be studied to further establish the true magnitude of these intriguing findings.\n                  <\/jats:p>","DOI":"10.1111\/sji.70094","type":"journal-article","created":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T01:44:51Z","timestamp":1769305491000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Similar Individual Serum Levels of\n                    <scp>MCP<\/scp>\n                    \u20101,\n                    <scp>TNF<\/scp>\n                    \u2010\u03b1, and\n                    <scp>IL<\/scp>\n                    \u20106 From Periodontitis Patients Before and 1\u2009Year After Treatment"],"prefix":"10.1111","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6464-5172","authenticated-orcid":false,"given":"Teun J.","family":"de Vries","sequence":"first","affiliation":[{"name":"Department of Periodontology, Academic Centre for Dentistry (ACTA) University of Amsterdam and Vrije Universiteit  Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5228-5998","authenticated-orcid":false,"given":"Sergio","family":"Bizzarro","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Academic Centre for Dentistry (ACTA) University of Amsterdam and Vrije Universiteit  Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5966-2186","authenticated-orcid":false,"given":"Irene","family":"Di Ceglie","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Radboud University  Nijmegen the Netherlands"},{"name":"IRCCS Human Research Hospital  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doran Y.","family":"Sol","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Academic Centre for Dentistry (ACTA) University of Amsterdam and Vrije Universiteit  Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter L. E. M.","family":"van Lent","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Radboud University  Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6649-374X","authenticated-orcid":false,"given":"Ton","family":"Schoenmaker","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Academic Centre for Dentistry (ACTA) University of Amsterdam and Vrije Universiteit  Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/08820139.2013.822766"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12002"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1385436"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12197"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00505"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/08916930701694717"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.591365"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198\u2010014\u20102779\u20101"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2024.1407396"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/life14070856"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13870"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12763"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20795"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24702"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology14030250"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u2010051X.2007.01173.x"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.2147\/PRBM.S384267"},{"issue":"1","key":"e_1_2_9_19_1","first-page":"9","article-title":"The Serum Levels of CCL2 and CCL16 Expression in Patients With Irritable Bowel Syndrome","volume":"8","author":"Hayatbakhsh M. 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