{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T08:03:04Z","timestamp":1784707384870,"version":"3.55.0"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Identifying sensitive and specific blood biomarkers for early warning of severe COVID\u201019 remains a critical clinical need. Based on previous findings of elevated cytokines in bronchoalveolar lavage fluid and blood, we developed a novel luciferase immunosorbent assay (LISA) that selectively quantifies biologically active full\u2010length suPAR and can eliminate interference from inactive fragments measured by conventional ELISA. Compared with conventional ELISA, LISA showed a lower detection limit (5.08 vs. 45.72\u2009pg\/mL) and a wider linear range (45.72\u2013100,000.00 vs. 1234.46\u201311,111.11\u2009pg\/mL). In adult COVID\u201019 patients, suPAR levels were significantly elevated in severe cases (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001) and correlated positively with NLR and\u00a0SII (\n                    <jats:italic>p<\/jats:italic>\n                    = 0.003 for each), and with IL\u20106 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001), but negatively with lymphocyte count (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.004). suPAR outperformed conventional inflammatory markers in predicting severe disease (AUC\u2009=\u20090.785) and adverse outcomes (AUC\u2009=\u20090.912), supporting its role as an independent predictor. In paediatric COVID\u201019 patients, suPAR remained superior to other single peripheral blood biomarkers for severity prediction, despite weaker correlations with inflammatory indices. This study establishes a reliable method for quantifying functionally intact suPAR and demonstrates its age\u2010dependent predictive value, highlighting its potential for individualized risk stratification in COVID\u201019 patients.\n                  <\/jats:p>","DOI":"10.1111\/sji.70130","type":"journal-article","created":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T14:58:38Z","timestamp":1782399518000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a Detection Method for Soluble Urokinase\u2010Type Plasminogen Activator Receptor and Its Application in Predicting Prognosis of Severe COVID\u201019"],"prefix":"10.1111","volume":"104","author":[{"given":"Yulin","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Epidemiology School of Public Health, Southern Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zebin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Epidemiology School of Public Health, Southern Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Epidemiology School of Public Health, Southern Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongliang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology The First People's Hospital of Chenzhou  Chenzhou Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shixing","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Epidemiology School of Public Health, Southern Medical University  Guangzhou Guangdong China"},{"name":"Institute of Global Health Southern Medical University  Guangzhou Guangdong China"},{"name":"Dermatology Hospital Southern Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2012-7"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40291-020-00481-8"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamainternmed.2020.0994"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-021-00656-2"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2025.1525051"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30566-3"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.medcle.2020.05.004"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25770"},{"issue":"2","key":"e_1_2_9_10_1","first-page":"852","article-title":"Soluble Urokinase Plasminogen Activator Receptor (suPAR) as a Prognostic Marker in 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