{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T05:26:41Z","timestamp":1787203601906,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100012774","name":"Innovationsfonden","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012774","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Th17\u2010cells are crucial for defence against fungal and bacterial infections and deficiency characterises certain inborn errors of immunity (IEI), including\n                    <jats:italic>STAT3<\/jats:italic>\n                    \u2010related Hyper IgE syndrome (HIES) and\n                    <jats:italic>STAT1<\/jats:italic>\n                    \u2010related Chronic Mucocutaneous Candidiasis (CMC). Standardised methods for clinical Th17\u2010cell quantification are limited. We developed a flow cytometric assay for quantifying Th17\u2010cells in peripheral blood based on PMA\/ionomycin stimulation and intracellular IL\u201017 labeling and evaluated its precision, robustness and longitudinal stability. A reference interval was established in 51 healthy adults and the impact of age and sex was assessed. The assay was applied to 31 patients carrying\n                    <jats:italic>STAT1<\/jats:italic>\n                    or\n                    <jats:italic>STAT3<\/jats:italic>\n                    variants or presenting phenotypes for which Th17\u2010cell quantification is informative. Diagnostic performance was analysed using receiver operating characteristic (ROC) curves and Matthew's Correlation Coefficients (MCC). The assay showed high intra\u2010 and inter\u2010assay precision (CV 5.1% and 10%) and Th17\u2010cell fractions remained stable after 72\u2009h of post\u2010fixation storage. Delayed pre\u2010fixation reduced precision. Age and sex did not significantly influence Th17\u2010cell fractions, supporting a unified Th17\u2010cell reference interval of T\u2010helper cells [0.21%\u20131.7%]. Compared with healthy controls, Th17\u2010cell fractions were significantly lower in patients with\n                    <jats:italic>STAT3<\/jats:italic>\n                    variants, patients with HIES or with both. ROC and MCC analyses indicated that Th17\u2010cell fractions above 0.44% reliably excluded pathogenic variants or associated phenotypes in all patient groups. This validated Th17\u2010cell assay is robust, reproducible and suitable for clinical use. It can guide genetic testing in IEI by identifying patients with Th17\u2010cell deficiencies and excluding variants or phenotypes unlikely to be causative.\n                  <\/jats:p>","DOI":"10.1111\/sji.70140","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T05:05:10Z","timestamp":1786683910000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["From Validation to Clinical Interpretation: A Flow Cytometric Th17\u2010Cell Assay for\n                    <scp>\n                      <i>STAT1<\/i>\n                    <\/scp>\n                    \u2010 and\n                    <scp>\n                      <i>STAT3<\/i>\n                    <\/scp>\n                    \u2010Related Inborn Errors of Immunity"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-6374-9806","authenticated-orcid":false,"given":"Marie Juhl","family":"Toft","sequence":"first","affiliation":[{"name":"Department of Clinical Immunology Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camilla Darum","family":"S\u00f8rensen","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5158-3670","authenticated-orcid":false,"given":"Mette","family":"Christiansen","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology Aarhus University Hospital  Aarhus Denmark"},{"name":"Department of Molecular Medicine Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3963-3804","authenticated-orcid":false,"given":"Carsten Schade","family":"Larsen","sequence":"additional","affiliation":[{"name":"Department of 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University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5989-9298","authenticated-orcid":false,"given":"Nicklas Heine","family":"Staunstrup","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2808-4755","authenticated-orcid":false,"given":"Bjarne Kuno","family":"M\u00f8ller","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6317-553X","authenticated-orcid":false,"given":"Mikkel Steen","family":"Petersen","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology Aarhus University Hospital  Aarhus 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