{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:07:51Z","timestamp":1790140071955,"version":"4.0.1"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study evaluated the diagnostic utility of eosinophil activation testing in allergic rhinitis (AR) by monitoring CD63 and Fc\u03b5RI expression on peripheral blood eosinophils before and after allergen stimulation. We enrolled 59 AR patients, 35 non\u2010allergic rhinitis (NAR) patients, and 19 healthy controls (HC), stratified by sIgE results and clinical symptoms. Eosinophils were identified via flow cytometry as CCR3+ cells. We quantified baseline eosinophil counts and CD63\/Fc\u03b5RI expression, and then measured their changes following stimulation with 0.1 and 1.0\u2009\u03bcg\/mL dust mite allergen. Diagnostic accuracy was assessed using ROC analysis and Spearman's correlation with sIgE as the reference standard. AR patients had higher baseline eosinophil counts and CD63 expression than NAR or HC (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Fc\u03b5RI was elevated in both AR and NAR relative to HC. Upon allergen stimulation, CD63 increased significantly only in AR, and this increase was dose\u2010dependent (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05); Fc\u03b5RI showed no response in any group. In this exploratory cohort, CD63 showed promising discriminatory ability, with an AUC of 0.899 (0.1\u2009\u03bcg\/mL) and 0.994 (1.0\u2009\u03bcg\/mL); a moderate positive correlation with sIgE was also observed (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009=\u20090.358\u20130.461). In contrast, Fc\u03b5RI performed around chance level (AUC 0.534\u20130.544). The eosinophil CD63 activation test can detect allergen\u2010specific responses in AR and shows a moderate positive correlation with sIgE, suggesting potential as a complementary tool for AR diagnosis.\n                  <\/jats:p>","DOI":"10.1111\/sji.70150","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T08:48:09Z","timestamp":1788943689000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Correlation Analysis Between Increased CD63 Expression Detected by Eosinophil Activation Test and sIgE Levels"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4976-6771","authenticated-orcid":false,"given":"Qianyun","family":"Li","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology and Head &amp; Neck Surgery Changping Hospital Affiliated to Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huagong","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology and Head and Neck Surgery, Shanghai Fourth People's Hospital, and School of Medicine Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2659-388X","authenticated-orcid":false,"given":"Zhao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Dongzhimen Hospital Beijing University of Chinese Medicine  Beijing China"},{"name":"Institute of Traditional Chinese Medicine Otorhinolaryngology Head and Neck Surgery, Dongzhimen Hospital Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengxia","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Medicine Shanghai Eighth People's Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/1744666X.2024.2336568"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12865-020-00395-4"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.16390"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/njcp.njcp_605_23"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.16390"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00431-026-06975-7"},{"issue":"11","key":"e_1_2_13_8_1","first-page":"1027","article-title":"The Diagnostic Value of Eosinophilic and Eosinophilic Cationic Proteins in Blood and Nasal Secretions for Allergic Rhinitis","volume":"33","author":"Yu W. T.","year":"2019","journal-title":"Journal of Clinical Otorhinolaryngology Head and Neck Surgery"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-025-03651-5"},{"issue":"1","key":"e_1_2_13_10_1","first-page":"41","article-title":"Using CD63 and CD203 in Diagnosing Food Allergies","volume":"31","author":"Irhayyif R. A.","year":"2024","journal-title":"Romanian Journal of Diabetes, Nutrition, and Metabolic Diseases"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1202323"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2072"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23020788"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-1016-8_8"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2021.02.013"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2024.107886"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.2147\/JAA.S282039"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.16242"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2024.155663"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.iac.2008.09.004"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.05.017"},{"key":"e_1_2_13_22_1","unstructured":"M. T. A.Kleuskens \u201cLocal Immune Responses in Eosinophilic Esophagitis: Implications for Disease Pathophysiology and Dietary Management (Doctoral Dissertation Utrecht University) \u201d(2024)."},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1159\/000053782"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.15930"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.waojou.2024.100998"},{"key":"e_1_2_13_26_1","first-page":"000","article-title":"Evaluation of the Clinical Performance of Multiple Serum sIgE Detection Systems Based on Component\u2010Resolved Diagnosis","volume":"44","author":"Huang Z.","year":"2024","journal-title":"Asian Pacific Journal of Allergy and Immunology"},{"key":"e_1_2_13_27_1","first-page":"215","volume-title":"Pediatric Airway Diseases","author":"\u00d6zdemiral C.","year":"2025"},{"key":"e_1_2_13_28_1","first-page":"29","volume-title":"Textbook of Pediatric Allergy","author":"Tosca M. A.","year":"2025"}],"container-title":["Scandinavian Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/sji.70150","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/sji.70150","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/sji.70150","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T04:56:24Z","timestamp":1790139384000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/sji.70150"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":27,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/sji.70150"],"URL":"https:\/\/doi.org\/10.1111\/sji.70150","archive":["Portico"],"relation":{},"ISSN":["0300-9475","1365-3083"],"issn-type":[{"value":"0300-9475","type":"print"},{"value":"1365-3083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-02-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70150"}}