{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T07:37:56Z","timestamp":1792741076975,"version":"4.5.0"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:00:00Z","timestamp":1791590400000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Exosomes derived from lung cancer cells play a critical role in modulating the tumour microenvironment, particularly through their effects on macrophage polarization. We investigated how exosomes from A549 lung adenocarcinoma cells, both unstimulated and stimulated with a toll\u2010like receptor 4 (TLR4) agonist, influence the phenotype and function of THP\u20101 derived macrophages. We used differential centrifugation and immunobead assay to isolate exosomes. Exosomes from unstimulated A549 cells promoted a mixed M1\/M2 macrophage phenotype with a bias towards M2 polarization, while exosomes from TLR4\u2010stimulated A549 cells reduced M2 marker expression without affecting M1 polarization. Both types of exosomes increased macrophage phagocytic capacity compared to PMA\u2010differentiated controls, but TLR4\u2010agonists did not increase it further. These findings provide new insights into how TLR agonist\u2010stimulated lung cancer\u2010derived exosomes shape macrophage phenotypes and functions, with implications for understanding tumour\u2010immune interactions and developing novel therapeutic strategies targeting the tumour microenvironment.<\/jats:p>","DOI":"10.1111\/sji.70159","type":"journal-article","created":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T08:17:35Z","timestamp":1791620255000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exosomes From Toll\u2010Like Receptor 4\u2010Stimulated Lung Cancer Cells Modulate Macrophage Polarization"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8130-422X","authenticated-orcid":false,"given":"Ba\u015fak","family":"Dalk\u0131ran","sequence":"first","affiliation":[{"name":"Department of Basic Oncology Hacettepe University Cancer Institute  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6933-1658","authenticated-orcid":false,"given":"Ranya","family":"Erdal","sequence":"additional","affiliation":[{"name":"Department of Basic Oncology Hacettepe University Cancer Institute  Ankara Turkey"},{"name":"Medical Scientist Training Program Hacettepe University Faculty of Medicine  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hande","family":"Canp\u0131nar","sequence":"additional","affiliation":[{"name":"Department of Basic Oncology Hacettepe University Cancer Institute  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21871"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-024-00551-9"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.115891"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2017.08.003"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2016.10.009"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.31774"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-021-01312-y"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-015-3630-9"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2011.04.009"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-020-01318-0"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01452-1"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.70670"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41698-023-00364-1"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2015.4044"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-04004-z"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.annonc.2024.10.006"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-020-00261-0"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.116014"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1033483"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.19105"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.07.068"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-020-01335-z"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s11658-022-00384-y"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9051303"},{"issue":"11","key":"e_1_2_10_26_1","first-page":"967","article-title":"Human THP\u20101 Macrophages Activated by Exosomes Derived From Lung Adenocarcinoma Cells Promote Lung Cancer Cell Invasion","volume":"35","author":"Chen L. 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