{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T16:16:24Z","timestamp":1789402584600,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,6,9]],"date-time":"2015-06-09T00:00:00Z","timestamp":1433808000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001322","name":"South African Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Scandinavian Med Sci Sports"],"published-print":{"date-parts":[[2016,6]]},"abstract":"\n High\u2010intensity exercise results in immune activation. This study determined whether (a) there is concordance between serum\n MPO<\/jats:styled-content>\n and neutrophil and\/or monocyte intracellular\n MPO<\/jats:styled-content>\n content; (b) peripheral blood mononuclear cells respond to inflammatory interleukins (\n ILs<\/jats:styled-content>\n ) by increasing intracellular signaling. Healthy male (\n n<\/jats:italic>\n \u2009=\u200912) volunteers participated in high\u2010intensity running (12\u2009\u00d7\u20095\u2009min, 10% decline, 15\u2009km\/h). Blood sample (pre, post, 4\u2009h) analyses included serum concentrations of\n IL<\/jats:styled-content>\n \u20101\u03b2,\n IL<\/jats:styled-content>\n \u20101ra,\n IL<\/jats:styled-content>\n \u20104,\n IL<\/jats:styled-content>\n \u20106,\n IL<\/jats:styled-content>\n \u20108,\n IL<\/jats:styled-content>\n \u201010, matrix metalloprotease\u20109 (\n MMP<\/jats:styled-content>\n \u20109) and creatine kinase (\n CK<\/jats:styled-content>\n ). Intracellular\n IL<\/jats:styled-content>\n \u20106,\n IL<\/jats:styled-content>\n \u201010,\n MPO<\/jats:styled-content>\n and\n STAT<\/jats:styled-content>\n 3\/\n SOCS<\/jats:styled-content>\n 3 signaling were assessed in mononuclear cells.\n CK<\/jats:styled-content>\n (1573\u2009\u00b1\u2009756\u2009u\/L),\n MMP<\/jats:styled-content>\n \u20109 (101\u2009\u00b1\u200927\u2009ng\/mL), neutrophil (9.89\u2009\u00b1\u20090.76\u2009\u00d7\u200910\n 9<\/jats:sup>\n cells\/L) and monocyte counts (1\u2009\u00b1\u20090.08\u2009\u00d7\u200910\n 9<\/jats:sup>\n cells\/L) increased at 4\u2009h. At 4\u2009h serum (7.1\u2009\u00b1\u20091.3\u2009ng\/mL) and monocyte\n MPO<\/jats:styled-content>\n (1.7\u2010fold) increased, whereas neutrophil\n MPO<\/jats:styled-content>\n decreased (0.8\u2010fold). Intracellular monocyte\n IL<\/jats:styled-content>\n \u201010 and\n IL<\/jats:styled-content>\n \u20106 decreased by 15% and 20\u201330%, respectively, coinciding with elevations in serum\n IL<\/jats:styled-content>\n \u201010 of 14.5\u2009\u00b1\u20094.7\u2009pg\/mL and\n IL<\/jats:styled-content>\n \u20106 of 5.4\u2009\u00b1\u20092.9\u2009pg\/mL, suggesting immune cell cytokine release in response to exercise. Intracellular\n PBMC<\/jats:styled-content>\n p\u2010\n STAT<\/jats:styled-content>\n 3 to total\n STAT<\/jats:styled-content>\n 3 ratio increased from pre to 4\u2009h. Circulating monocytes are responsive to increased serum\n IL<\/jats:styled-content>\n \u20106 suggesting a negative feedback loop via\n STAT<\/jats:styled-content>\n 3 signaling.\n <\/jats:p>","DOI":"10.1111\/sms.12497","type":"journal-article","created":{"date-parts":[[2015,6,10]],"date-time":"2015-06-10T20:53:38Z","timestamp":1433969618000},"page":"638-647","source":"Crossref","is-referenced-by-count":20,"title":["Neutrophil and monocyte responses to downhill running: Intracellular contents of\n MPO<\/scp>\n ,\n IL<\/scp>\n \u20106,\n IL<\/scp>\n \u201010, pstat3, and\n SOCS<\/scp>\n 3"],"prefix":"10.1111","volume":"26","author":[{"given":"M.","family":"van de Vyver","sequence":"first","affiliation":[{"name":"Department of Physiological Sciences Stellenbosch University Stellenbosch South Africa"},{"name":"Division of Endocrinology, Department of Medicine Stellenbosch University Tygerberg South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Engelbrecht","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences Stellenbosch University Stellenbosch South Africa"},{"name":"Central Analytical Facility Stellenbosch University Stellenbosch South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences Stellenbosch University Stellenbosch South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. H.","family":"Myburgh","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences Stellenbosch University Stellenbosch South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,6,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12410-014-9258-0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2010.04.008"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.3.1612"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.12.6404"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-011-1923-2"},{"issue":"1","key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.3233\/CH-2010-1361","article-title":"Effect of strenuous physical exercise on circulating cell\u2010derived microparticles","volume":"47","author":"Chaar V","year":"2011","journal-title":"Clin Hemorheol Microcirc"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00316.2004"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2014.01.014"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2014.01.021"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1747-1028-5-14"},{"key":"e_1_2_7_12_1","first-page":"72","article-title":"Eccentric exercise\u2010induced delayed\u2010onset muscle soreness and changes in markers of muscle damage and inflammation","volume":"19","author":"Kanda K","year":"2013","journal-title":"Exerc Immunol Rev"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0b013e3182a4e754"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-002-0755-5"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.5.2623"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-010-1572-x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00143.2013"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.051912"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.6.3263"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1249\/01.mss.0000177064.65927.98"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0b013e3181ac7adb"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01331.2003"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1249\/01.MSS.0000161804.05399.3B"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00121.2009"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00872.2007"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.2012.02720.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12166"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-971941"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1139\/h06-106"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00735.2009"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017392"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/sms.12164"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddq523"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.419788"}],"container-title":["Scandinavian Journal of Medicine & Science in Sports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fsms.12497","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/sms.12497","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T21:32:44Z","timestamp":1696195964000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/sms.12497"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6,9]]},"references-count":33,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["10.1111\/sms.12497"],"URL":"https:\/\/doi.org\/10.1111\/sms.12497","archive":["Portico"],"relation":{},"ISSN":["0905-7188","1600-0838"],"issn-type":[{"value":"0905-7188","type":"print"},{"value":"1600-0838","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,9]]}}}