{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:22:09Z","timestamp":1787016129682,"version":"build-2736575974"},"reference-count":55,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,8,13]],"date-time":"2021-08-13T00:00:00Z","timestamp":1628812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scandinavian Med Sci Sports"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:p>\n                    Injuries to the intra\u2010articular anterior cruciate ligament (ACL) and the extra\u2010articular medial collateral ligament (MCL) result in significant knee joint instability, pain, and immobility. Moderate endurance\u2010type exercise can increase ligament strength but little is known on the effect of short\u2010term regular bouts of high\u2010intensity exercise on the extracellular matrix (ECM) structure of knee ligaments. Therefore, this study aimed to identify the effect of short\u2010term regular bouts high exercise on the proteome of the rat ACL and MCL using mass spectrometry. Sprague\u2010Dawley male rats (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a06) were split into control and exercise groups, and subjected to high\u2010intensity training for four 4\u00a0weeks followed by proteomic analyses of the ACL and MCL. Knee joint health status was assessed using OARSI and a validated histological scoring system. Histopathological analyses demonstrated no significant changes in either in cruciate, collateral ligaments, or cartilage between the control and exercised knee joints. However, significant proteins were found to be more abundant in the exercised ACL compared to ACL control group but not between the exercised MCL and control MCL groups. The significant abundant proteins in ACL exercise groups were mostly cytoskeletal, ribosomal and enzymes with several abundant matrisomal proteins such as collagen proteins and proteoglycans being found in this group. In conclusion, our results indicate that short\u2010term regular bouts of high\u2010intensity exercise have an impact on the intra\u2010articular ACL but not extra\u2010articular MCL ECM protein expression.\n                  <\/jats:p>","DOI":"10.1111\/sms.14023","type":"journal-article","created":{"date-parts":[[2021,7,16]],"date-time":"2021-07-16T12:15:42Z","timestamp":1626437742000},"page":"2033-2043","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The effect of exercise on the protein profile of rat knee joint intra\u2010 and extra\u2010articular ligaments"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4925-0206","authenticated-orcid":false,"given":"Yalda A.","family":"Kharaz","sequence":"first","affiliation":[{"name":"Department of Musculoskeletal and Ageing Sciences Institute of Life Course and Medical Sciences University of Liverpool Liverpool 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