{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T12:52:00Z","timestamp":1785761520421,"version":"3.56.0"},"reference-count":41,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T00:00:00Z","timestamp":1783728000000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009059","name":"Defence Science Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009059","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scandinavian Med Sci Sports"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Quadriceps muscle deficits following anterior cruciate ligament (ACL) injury can persist years beyond reconstruction (ACLR), but the underlying molecular mechanisms remain largely unexplored. This study investigated whether persistent muscle deficits following ACLR are associated with defects in hypertrophic signaling pathways in response to acute resistance exercise. Twelve participants (7 females, 5 males; age 26.7\u2009\u00b1\u20094.1\u2009years; 2.6\u2009\u00b1\u20091.5\u2009years post\u2010ACL injury) underwent MRI scanning and one\u2010repetition maximum (1RM) testing to determine muscle volume and unilateral leg\u2010press strength in the ACLR versus contralateral control leg. Following a standardized meal and overnight fast, participants performed unilateral leg\u2010press resistance exercise (6 sets of 8 repetitions at 80% 1RM). Vastus lateralis (VL) muscle biopsies were collected from both legs at rest and immediately post\u2010exercise. Immunoblotting assessed phosphorylation status and total protein content of hypertrophic signaling proteins (e.g., mTORC1 pathway). Total knee extensor muscle volume (7.5% deficit;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.037), VL (7.7% deficit;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.031), and rectus femoris (5.8% deficit;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.022) muscle volumes were decreased in the ACLR versus contralateral control leg. Knee extensor (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009=\u20090.37;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.036) and VL (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009=\u20090.36;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.038) deficits correlated with increased time since injury, with no associated unilateral leg press 1RM strength difference between legs (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.192). Despite main effects of exercise observed for mTORC1 pathway signaling proteins (i.e., decreased p\u2010TSC2 Thr1462;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.002 and p\u20104E\u2010BP1 Thr37\/46;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001, and increased p\u2010S6 Ser240\/244;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.032), no between\u2010leg differences in mTORC1 signaling were detected at rest or immediately post\u2010exercise under the specific experimental conditions tested. In conclusion, persistent muscle volume deficits following ACLR were less pronounced with increased time since injury but were not associated with detectable differences between legs in unilateral leg\u2010press strength. The lack of detectable between\u2010leg differences in vastus lateralis mTORC1 signaling warrants further investigation at timepoints closer to ACLR and\/or later post\u2010exercise.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Trial Registration:<\/jats:bold>\n                    This trial was prospectively registered at the Australian New Zealand Clinical Trials Registry (ANZCTR; trial registration number 12622001166763)\n                  <\/jats:p>","DOI":"10.1111\/sms.70343","type":"journal-article","created":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T07:19:44Z","timestamp":1783754384000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Persistent Human Quadriceps Muscle Volume Deficits Following\n                    <scp>ACL<\/scp>\n                    Reconstruction Are Associated With No Detectable Between\u2010Leg Differences in Vastus Lateralis Muscle Signaling at Rest or Immediately Following Resistance Exercise"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-7359-4826","authenticated-orcid":false,"given":"Miriam J.","family":"Smith","sequence":"first","affiliation":[{"name":"School of Behavioural and Health Sciences Australian Catholic University  Fitzroy Victoria Australia"},{"name":"Sports Performance, Recovery, Injury and New Technologies (SPRINT) Research Centre Australian Catholic University  Fitzroy Victoria Australia"},{"name":"Centre for Human Metabolism and Performance, Mary MacKillop Institute for Health Research Australian Catholic University  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3082-6236","authenticated-orcid":false,"given":"Mehdi R.","family":"Belhaj","sequence":"additional","affiliation":[{"name":"Centre for Human Metabolism and Performance, Mary MacKillop Institute for Health Research Australian Catholic University  Melbourne Victoria Australia"},{"name":"Centaur Institute  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4964-1848","authenticated-orcid":false,"given":"Ryan G.","family":"Timmins","sequence":"additional","affiliation":[{"name":"School of Behavioural and Health Sciences Australian Catholic University  Fitzroy Victoria Australia"},{"name":"Sports Performance, Recovery, Injury and New Technologies (SPRINT) Research Centre Australian Catholic University  Fitzroy Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6180-6003","authenticated-orcid":false,"given":"Nirav","family":"Maniar","sequence":"additional","affiliation":[{"name":"School of Behavioural and Health Sciences Australian Catholic University  Fitzroy Victoria Australia"},{"name":"Sports Performance, Recovery, Injury and New Technologies (SPRINT) Research Centre Australian Catholic University  Fitzroy Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy S.","family":"Whitehead","sequence":"additional","affiliation":[{"name":"Sports Performance, Recovery, Injury and New Technologies (SPRINT) Research Centre Australian Catholic University  Fitzroy Victoria Australia"},{"name":"Centaur Institute  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8354-6353","authenticated-orcid":false,"given":"David A.","family":"Opar","sequence":"additional","affiliation":[{"name":"School of Behavioural and Health Sciences Australian Catholic University  Fitzroy Victoria Australia"},{"name":"Sports Performance, Recovery, Injury and New Technologies (SPRINT) Research Centre Australian Catholic University  Fitzroy Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5168-7082","authenticated-orcid":false,"given":"Nolan J.","family":"Hoffman","sequence":"additional","affiliation":[{"name":"Centre for Human Metabolism and Performance, Mary MacKillop Institute for Health Research Australian Catholic University  Melbourne Victoria 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