{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T16:54:17Z","timestamp":1761929657625},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,3,28]],"date-time":"2013-03-28T00:00:00Z","timestamp":1364428800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:sec><jats:title>Background\/Aims<\/jats:title><jats:p>The purpose of the present pilot study was to investigate the feasibility of combining large pore dermal microdialysis with shotgun proteomic analysis in human skin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Dialysate was recovered from human skin by 2000 <jats:styled-content style=\"fixed-case\">kD<\/jats:styled-content>a microdialysis membranes from one subject at three different phases of the study; trauma due to implantation of the dialysis device, a post implantation steady\u2010state period, and after induction of vasodilatation and plasma extravasation. For shotgun proteomics, the proteins were extracted and digested with trypsin. Peptides were separated by capillary and nanoflow <jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content> systems, followed by tandem mass spectrometry (<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>) on a Quadrupole\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content> hybrid instrument. The <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> spectra were merged and mapped to a human target protein database to achieve peptide identification and protein inference.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Results showed variation in protein amounts and profiles for each of the different sampling phases. The total protein concentration was 1.7, 0.6, and 1.3\u00a0mg\/<jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content> during the three phases, respectively. A total of 158 different proteins were identified. Immunoglobulins and the major classes of plasma proteins, including proteases, coagulation factors, apolipoproteins, albumins, and complement factors, make up the major load of proteins in all three test conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Shotgun proteomics allowed the identification of more than 150 proteins in microdialysis samples from human skin. This highlights the opportunities of <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> to study the complex molecular interactions in the skin.<\/jats:p><\/jats:sec>","DOI":"10.1111\/srt.12063","type":"journal-article","created":{"date-parts":[[2013,3,28]],"date-time":"2013-03-28T15:47:16Z","timestamp":1364485636000},"page":"424-431","source":"Crossref","is-referenced-by-count":9,"title":["Large pore dermal microdialysis and liquid chromatography\u2010tandem mass spectroscopy shotgun proteomic analysis: a feasibility study"],"prefix":"10.1111","volume":"19","author":[{"given":"Lars J.","family":"Petersen","sequence":"first","affiliation":[{"name":"Department of Nuclear Medicine Aalborg University Hospital Aalborg Denmark"},{"name":"Department of Health Sciences and Technology Aalborg University Aalborg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mette A.","family":"S\u00f8rensen","sequence":"additional","affiliation":[{"name":"Department of Large Animal Sciences Faculty of Health and Medical Sciences University of Copenhagen Taastrup Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marius C.","family":"Codrea","sequence":"additional","affiliation":[{"name":"Department of Animal Science Faculty of Science and Technology Aarhus University Aarhus Denmark"},{"name":"Department of Molecular Biology and Genetics Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helle D.","family":"Zacho","sequence":"additional","affiliation":[{"name":"Department of Clinical Physiology Viborg Hospital Viborg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Em\u00f8ke","family":"Bendixen","sequence":"additional","affiliation":[{"name":"Department of Animal Science Faculty of Science 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