{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T01:57:25Z","timestamp":1777514245431,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,6,25]],"date-time":"2013-06-25T00:00:00Z","timestamp":1372118400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Visual clinical methods of skin color evaluation for diagnostic purposes are so far mostly subjective and thus inaccurate. We present a modified method of melanin amount measurement based on diffuse reflectance spectroscopy (<jats:styled-content style=\"fixed-case\">DRS<\/jats:styled-content>). This method is non\u2010invasive and objective, and allows easy quantification and comparison of melanin levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Skin pigmentation was measured by <jats:styled-content style=\"fixed-case\">DRS<\/jats:styled-content> method in 0\u201018 year old patients at the Department of Pediatric Dermatovenerology, School of Medicine Comenius University Bratislava. Patients were treated for their vitiligo by Balneo <jats:styled-content style=\"fixed-case\">PUVA<\/jats:styled-content> treatment twice weekly. Each patient had measured his remittance spectra from the treated vitiliginous skin before the treatment was started, after 10 irradiations of Balneo <jats:styled-content style=\"fixed-case\">PUVA<\/jats:styled-content> and at the end of the treatment after 25 irradiations of Balneo <jats:styled-content style=\"fixed-case\">PUVA<\/jats:styled-content>. In our study as a reference skin for spectroscopic assessment of melanin <jats:italic>in vivo<\/jats:italic> was used the averaged remittance spectra (measured on the inner arm) from the sample of 10 albino patients. The remittance spectra obtained from the vitiligo patients were ratioed against the newly described remittance reference albino skin. We exploited the linear behavior of the spectral curve in the 620\u2010720 nm interval (significant for melanin absorption) and used the slope of the regression line to compute the quantification index \u03b1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>By clinical examination before the Balneo <jats:styled-content style=\"fixed-case\">PUVA<\/jats:styled-content> therapy, after the 10th dose of Balneo <jats:styled-content style=\"fixed-case\">PUVA<\/jats:styled-content> therapy as well as at the end of the complete course of Balneo <jats:styled-content style=\"fixed-case\">PUVA<\/jats:styled-content> therapy (after 25 irradiations) we recorded a marked increase of pigmentation in all treated patients for their vitiligo. In each patient the values of melanin quantification angle \u03b1 were calculated. Statistically we found a significant difference between the melanin quantification angle \u03b1 in vitiliginous skin before, during the 10th dose of treatment and after the treatment. Similar significant difference was also observed between treated and non\u2010involved skin. We could confirm a clear association between clinical visual examination of treated vitiligo lesions, objective data collected by <jats:styled-content style=\"fixed-case\">DRS<\/jats:styled-content> and melanin quantification angle \u03b1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>By using a new standard for the reference skin (albino skin) we could more exactly compare melanin levels in different subjects. Our proposed melanin quantification angle \u03b1 expresses the extent of the difference in melanin levels between the examined skin lesions. We successfully used this index to quantify the variations of melanin (progress of repigmentation) throughout different stages of treatment of the same lesion and also to objectively evaluate the final effect of the therapy. In the present study, we showed that the diffuse reflectance spectroscopy (<jats:styled-content style=\"fixed-case\">DRS<\/jats:styled-content>) may be suitable method to measure skin colour and the content of human skin melanin <jats:italic>in vivo<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/srt.12092","type":"journal-article","created":{"date-parts":[[2013,6,25]],"date-time":"2013-06-25T14:53:53Z","timestamp":1372172033000},"page":"108-115","source":"Crossref","is-referenced-by-count":8,"title":["An objective assessment of melanin in vitiligo skin treated with Balneo <scp>PUVA<\/scp> therapy"],"prefix":"10.1111","volume":"20","author":[{"given":"V.","family":"Hegyi","sequence":"first","affiliation":[{"name":"Department of Pediatric Dermatovenerology Comenius University Bratislava Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Petrovajov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dermatovenerology Comenius University Bratislava Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Novotn\u00fd","sequence":"additional","affiliation":[{"name":"Faculty of Mathematics Physics and Informatics Comenius University Bratislava Slovak Republic"},{"name":"VIS GRAVIS, s.r.o. Bratislava Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,6,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0749.2006.00355.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12492607"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.6.1203"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00105-009-1915-x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0781.2006.00195.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1990.tb01969.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1468-3083.2002.00407.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470987100.ch28"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2012.04505.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI100243"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1000650102"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1991.tb08470.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2002.120790"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0749.2004.00204.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.1647545"},{"key":"e_1_2_7_17_1","first-page":"109","article-title":"Diffuse reflectance spectrophotometry I. spectrophotometer and its engineering solution","volume":"84","author":"Sor\u0161\u00e1kov\u00e1 Trnovsk\u00e1 K","year":"2009","journal-title":"\u010ces\u2010slov Derm"},{"key":"e_1_2_7_18_1","first-page":"21","article-title":"Diffuse reflectance spectrophotometry II. practical applications \u2010 a pilot study","volume":"85","author":"Sor\u0161\u00e1kov\u00e1 Trnovsk\u00e1 K","year":"2010","journal-title":"\u010ces\u2010slov Derm"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12479191"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1088\/0031-9155\/45\/3\/312"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/ics.12003"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0022-202x.2001.01630.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2008.08642.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.1961.01580080079009"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1088\/0031-9155\/25\/4\/008"},{"key":"e_1_2_7_26_1","first-page":"249","article-title":"Noninvasive computerized analysis of skin chromophores in vivo by reflectance spectroscopy","volume":"7","author":"Andersen PH","year":"1990","journal-title":"Photodermatol Photoimmunol Photomed"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12275011"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1986.tb05590.x"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.12170"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2002.19635.x"}],"container-title":["Skin Research and Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fsrt.12092","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/srt.12092","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T15:42:27Z","timestamp":1696174947000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/srt.12092"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6,25]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,2]]}},"alternative-id":["10.1111\/srt.12092"],"URL":"https:\/\/doi.org\/10.1111\/srt.12092","archive":["Portico"],"relation":{},"ISSN":["0909-752X","1600-0846"],"issn-type":[{"value":"0909-752X","type":"print"},{"value":"1600-0846","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6,25]]}}}