{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T18:16:26Z","timestamp":1779214586547,"version":"3.51.4"},"reference-count":10,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,12,23]],"date-time":"2014-12-23T00:00:00Z","timestamp":1419292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"LEO Pharma"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Vascular modifications represent a key feature in psoriatic plaques. Previous research with Laser Doppler Perfusion Imaging (<jats:styled-content style=\"fixed-case\">LDPI<\/jats:styled-content>) revealed a remarkable heterogeneity in the cutaneous perfusion within homogenous\u2010appearing psoriatic lesions. Insights in the relation between perfusion during treatment and related biological changes are lacking.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To study the effect of calcipotriol\u2010betamethasone dipropionate ointment on the microcirculation and the expression levels of immunohistochemical markers in psoriatic lesions compared to the distant uninvolved skin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Psoriatic lesions of fourteen patients were treated once a day during 8\u00a0weeks. Clinical SUM scores and the perfusion intensity by means of <jats:styled-content style=\"fixed-case\">LDPI<\/jats:styled-content> were assessed every 2\u00a0weeks. After 8\u00a0weeks, a biopsy from the target lesion and one from the distant uninvolved skin were taken and stained for psoriasis\u2010related markers, like <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017 and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>31.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After 8\u00a0weeks, seven patients reached a SUM score of 0 or 1, and were classified as good\u2010responders. The other patients were classified moderate\u2010responders. The perfusion intensity decreased in all lesions during therapy. In the good\u2010responders, all investigated psoriasis\u2010related proteins within the treated lesions reached the expression level found within the distant uninvolved skin. The expression of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>31, however, was significantly higher in the treated lesions as compared to the distant uninvolved skin (<jats:italic>p\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.0156). In the moderate responders, almost all expression levels remained significantly elevated compared to the uninvolved skin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In the skin of good\u2010responders the expression of dermal <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>31<jats:sup>+<\/jats:sup> endothelium remains significantly elevated within the treated lesions compared with the distant uninvolved skin, whereas a marked reduction in the perfusion intensity and SUM score was found. This indicates that clinical improvement might outrun endothelial changes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/srt.12198","type":"journal-article","created":{"date-parts":[[2014,12,23]],"date-time":"2014-12-23T10:34:04Z","timestamp":1419330844000},"page":"340-345","source":"Crossref","is-referenced-by-count":15,"title":["Clearing of psoriasis documented by laser Doppler perfusion imaging contrasts remaining elevation of dermal expression levels of <scp>CD<\/scp>31"],"prefix":"10.1111","volume":"21","author":[{"given":"A. G. M.","family":"Hendriks","sequence":"first","affiliation":[{"name":"Department of Dermatology Radboud University Medical Center Enschede The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. C. M.","family":"van de Kerkhof","sequence":"additional","affiliation":[{"name":"Department of Dermatology Radboud University Medical Center Enschede The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. S.","family":"de Jonge","sequence":"additional","affiliation":[{"name":"Biomedical Photonic Imaging MIRA Institute for Biomedical Technology and Technical Medicine University of Twente Enschede The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Lucas","sequence":"additional","affiliation":[{"name":"Biomedical Photonic Imaging MIRA Institute for Biomedical Technology and Technical Medicine University of Twente Enschede The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Steenbergen","sequence":"additional","affiliation":[{"name":"Biomedical Photonic Imaging MIRA Institute for Biomedical Technology and Technical Medicine University of Twente Enschede The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. M. B.","family":"Seyger","sequence":"additional","affiliation":[{"name":"Department of Dermatology Radboud University Medical Center Enschede The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,12,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2613.2009.00669.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2009.04287.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/672381"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/09546634.2013.777382"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.17.003211"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12696791"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1994.tb08582.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2005.06479.x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1987.tb04116.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000354119"}],"container-title":["Skin Research and Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fsrt.12198","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/srt.12198","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T07:24:35Z","timestamp":1695540275000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/srt.12198"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,23]]},"references-count":10,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["10.1111\/srt.12198"],"URL":"https:\/\/doi.org\/10.1111\/srt.12198","archive":["Portico"],"relation":{},"ISSN":["0909-752X","1600-0846"],"issn-type":[{"value":"0909-752X","type":"print"},{"value":"1600-0846","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,23]]}}}